Cargando…
Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress
Cellular stress induces the formation of membraneless protein condensates in both the nucleus and cytoplasm. The nucleocytoplasmic transport of proteins mainly occurs through nuclear pore complexes (NPCs), whose efficiency is affected by various stress conditions. Here, we report that hyperosmotic s...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467775/ https://www.ncbi.nlm.nih.gov/pubmed/34572142 http://dx.doi.org/10.3390/cells10092493 |
_version_ | 1784573485550403584 |
---|---|
author | Lee, Jeeyoung Le, Ly Thi Huong Luu Kim, Eunkyoung Lee, Min Jae |
author_facet | Lee, Jeeyoung Le, Ly Thi Huong Luu Kim, Eunkyoung Lee, Min Jae |
author_sort | Lee, Jeeyoung |
collection | PubMed |
description | Cellular stress induces the formation of membraneless protein condensates in both the nucleus and cytoplasm. The nucleocytoplasmic transport of proteins mainly occurs through nuclear pore complexes (NPCs), whose efficiency is affected by various stress conditions. Here, we report that hyperosmotic stress compartmentalizes nuclear 26S proteasomes into dense nuclear foci, independent of signaling cascades. Most of the proteasome foci were detected between the condensed chromatin mass and inner nuclear membrane. The proteasome-positive puncta were not colocalized with other types of nuclear bodies and were reversibly dispersed when cells were returned to the isotonic medium. The structural integrity of 26S proteasomes in the nucleus was slightly affected under the hyperosmotic condition. We also found that these insulator-body-like proteasome foci were possibly formed through disrupted nucleus-to-cytosol transport, which was mediated by the sequestration of NPC components into osmostress-responding stress granules. These data suggest that phase separation in both the nucleus and cytosol may be a major cell survival mechanism during hyperosmotic stress conditions. |
format | Online Article Text |
id | pubmed-8467775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84677752021-09-27 Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress Lee, Jeeyoung Le, Ly Thi Huong Luu Kim, Eunkyoung Lee, Min Jae Cells Article Cellular stress induces the formation of membraneless protein condensates in both the nucleus and cytoplasm. The nucleocytoplasmic transport of proteins mainly occurs through nuclear pore complexes (NPCs), whose efficiency is affected by various stress conditions. Here, we report that hyperosmotic stress compartmentalizes nuclear 26S proteasomes into dense nuclear foci, independent of signaling cascades. Most of the proteasome foci were detected between the condensed chromatin mass and inner nuclear membrane. The proteasome-positive puncta were not colocalized with other types of nuclear bodies and were reversibly dispersed when cells were returned to the isotonic medium. The structural integrity of 26S proteasomes in the nucleus was slightly affected under the hyperosmotic condition. We also found that these insulator-body-like proteasome foci were possibly formed through disrupted nucleus-to-cytosol transport, which was mediated by the sequestration of NPC components into osmostress-responding stress granules. These data suggest that phase separation in both the nucleus and cytosol may be a major cell survival mechanism during hyperosmotic stress conditions. MDPI 2021-09-21 /pmc/articles/PMC8467775/ /pubmed/34572142 http://dx.doi.org/10.3390/cells10092493 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Jeeyoung Le, Ly Thi Huong Luu Kim, Eunkyoung Lee, Min Jae Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title | Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title_full | Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title_fullStr | Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title_full_unstemmed | Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title_short | Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress |
title_sort | formation of non-nucleoplasmic proteasome foci during the late stage of hyperosmotic stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467775/ https://www.ncbi.nlm.nih.gov/pubmed/34572142 http://dx.doi.org/10.3390/cells10092493 |
work_keys_str_mv | AT leejeeyoung formationofnonnucleoplasmicproteasomefociduringthelatestageofhyperosmoticstress AT lelythihuongluu formationofnonnucleoplasmicproteasomefociduringthelatestageofhyperosmoticstress AT kimeunkyoung formationofnonnucleoplasmicproteasomefociduringthelatestageofhyperosmoticstress AT leeminjae formationofnonnucleoplasmicproteasomefociduringthelatestageofhyperosmoticstress |