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The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was obs...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467794/ https://www.ncbi.nlm.nih.gov/pubmed/34572647 http://dx.doi.org/10.3390/antibiotics10091063 |
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author | Mišić, Dušan Kiskaroly, Ferenc Szostak, Michael P. Cabal, Adriana Ruppitsch, Werner Bernreiter-Hofer, Tanja Milovanovic, Viktoria Feßler, Andrea T. Allerberger, Franz Spergser, Joachim Müller, Elke Schwarz, Stefan Braun, Sascha D. Monecke, Stefan Ehricht, Ralf Korus, Maciej Benković, Damir Korzeniowska, Malgorzata Loncaric, Igor |
author_facet | Mišić, Dušan Kiskaroly, Ferenc Szostak, Michael P. Cabal, Adriana Ruppitsch, Werner Bernreiter-Hofer, Tanja Milovanovic, Viktoria Feßler, Andrea T. Allerberger, Franz Spergser, Joachim Müller, Elke Schwarz, Stefan Braun, Sascha D. Monecke, Stefan Ehricht, Ralf Korus, Maciej Benković, Damir Korzeniowska, Malgorzata Loncaric, Igor |
author_sort | Mišić, Dušan |
collection | PubMed |
description | The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003–0.00006% for chlorohexidine, of 4–6% for isopropanol and of 0.001–0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans. |
format | Online Article Text |
id | pubmed-8467794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84677942021-09-27 The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia Mišić, Dušan Kiskaroly, Ferenc Szostak, Michael P. Cabal, Adriana Ruppitsch, Werner Bernreiter-Hofer, Tanja Milovanovic, Viktoria Feßler, Andrea T. Allerberger, Franz Spergser, Joachim Müller, Elke Schwarz, Stefan Braun, Sascha D. Monecke, Stefan Ehricht, Ralf Korus, Maciej Benković, Damir Korzeniowska, Malgorzata Loncaric, Igor Antibiotics (Basel) Article The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003–0.00006% for chlorohexidine, of 4–6% for isopropanol and of 0.001–0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans. MDPI 2021-09-03 /pmc/articles/PMC8467794/ /pubmed/34572647 http://dx.doi.org/10.3390/antibiotics10091063 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mišić, Dušan Kiskaroly, Ferenc Szostak, Michael P. Cabal, Adriana Ruppitsch, Werner Bernreiter-Hofer, Tanja Milovanovic, Viktoria Feßler, Andrea T. Allerberger, Franz Spergser, Joachim Müller, Elke Schwarz, Stefan Braun, Sascha D. Monecke, Stefan Ehricht, Ralf Korus, Maciej Benković, Damir Korzeniowska, Malgorzata Loncaric, Igor The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title | The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title_full | The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title_fullStr | The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title_full_unstemmed | The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title_short | The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia |
title_sort | first report of mcr-1-carrying escherichia coli originating from animals in serbia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467794/ https://www.ncbi.nlm.nih.gov/pubmed/34572647 http://dx.doi.org/10.3390/antibiotics10091063 |
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