Cargando…

The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia

The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was obs...

Descripción completa

Detalles Bibliográficos
Autores principales: Mišić, Dušan, Kiskaroly, Ferenc, Szostak, Michael P., Cabal, Adriana, Ruppitsch, Werner, Bernreiter-Hofer, Tanja, Milovanovic, Viktoria, Feßler, Andrea T., Allerberger, Franz, Spergser, Joachim, Müller, Elke, Schwarz, Stefan, Braun, Sascha D., Monecke, Stefan, Ehricht, Ralf, Korus, Maciej, Benković, Damir, Korzeniowska, Malgorzata, Loncaric, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467794/
https://www.ncbi.nlm.nih.gov/pubmed/34572647
http://dx.doi.org/10.3390/antibiotics10091063
_version_ 1784573491383631872
author Mišić, Dušan
Kiskaroly, Ferenc
Szostak, Michael P.
Cabal, Adriana
Ruppitsch, Werner
Bernreiter-Hofer, Tanja
Milovanovic, Viktoria
Feßler, Andrea T.
Allerberger, Franz
Spergser, Joachim
Müller, Elke
Schwarz, Stefan
Braun, Sascha D.
Monecke, Stefan
Ehricht, Ralf
Korus, Maciej
Benković, Damir
Korzeniowska, Malgorzata
Loncaric, Igor
author_facet Mišić, Dušan
Kiskaroly, Ferenc
Szostak, Michael P.
Cabal, Adriana
Ruppitsch, Werner
Bernreiter-Hofer, Tanja
Milovanovic, Viktoria
Feßler, Andrea T.
Allerberger, Franz
Spergser, Joachim
Müller, Elke
Schwarz, Stefan
Braun, Sascha D.
Monecke, Stefan
Ehricht, Ralf
Korus, Maciej
Benković, Damir
Korzeniowska, Malgorzata
Loncaric, Igor
author_sort Mišić, Dušan
collection PubMed
description The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003–0.00006% for chlorohexidine, of 4–6% for isopropanol and of 0.001–0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans.
format Online
Article
Text
id pubmed-8467794
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84677942021-09-27 The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia Mišić, Dušan Kiskaroly, Ferenc Szostak, Michael P. Cabal, Adriana Ruppitsch, Werner Bernreiter-Hofer, Tanja Milovanovic, Viktoria Feßler, Andrea T. Allerberger, Franz Spergser, Joachim Müller, Elke Schwarz, Stefan Braun, Sascha D. Monecke, Stefan Ehricht, Ralf Korus, Maciej Benković, Damir Korzeniowska, Malgorzata Loncaric, Igor Antibiotics (Basel) Article The aim of this study was continuous monitoring of the presence of mcr-1 to mcr-5 genes in Enterobacterales isolated from cattle, pigs, and domestic poultry at intensive breeding facilities in Northern Vojvodina, Serbia, from 1 January 1 to 1 October 2020. Out of 2167 examined samples, mcr-1 was observed in five E. coli isolates originating from healthy turkeys. Four isolates belonged to the phylogenetic group B1, and one isolate to the phylogenetic group A. Detected E. coli serogenotypes (somatic O and flagellar H antigens) were O8:H25 and O29:H25. Core-genome multi-locus sequence typing (cgMLST) revealed three ST58 isolates clustering together in Clonal Complex (CC) 155 and two singletons of ST641-CC86 and ST410-CC23, respectively. Clonotyping revealed CH4-32 (n = 3), CH6-53 (n = 1) and CH4-24 (n = 1). In all isolates, the mcr-1 gene was located on a large IncX4 replicon type plasmid. Eight virulence-associated genes (VAGs) typical of avian pathogenic E. coli (APEC) (fyuA, fimH, hlyF, iss, ompT, sitA, traT, iroN) were detected in four isolates. These isolates were investigated for susceptibility to four biocides and revealed MIC values of 0.125% for glutardialdehyde, of 0.00003–0.00006% for chlorohexidine, of 4–6% for isopropanol and of 0.001–0.002% for benzalkonium chloride. All obtained MIC values of the tested biocides were comparable to the reference strain, with no indication of possible resistance. This is the first report of mcr-1.1-carrying E. coli from Serbia. Although only samples from turkeys were mcr-positive in this study, continuous monitoring of livestock samples is advised to prevent a spill-over from animals to humans. MDPI 2021-09-03 /pmc/articles/PMC8467794/ /pubmed/34572647 http://dx.doi.org/10.3390/antibiotics10091063 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mišić, Dušan
Kiskaroly, Ferenc
Szostak, Michael P.
Cabal, Adriana
Ruppitsch, Werner
Bernreiter-Hofer, Tanja
Milovanovic, Viktoria
Feßler, Andrea T.
Allerberger, Franz
Spergser, Joachim
Müller, Elke
Schwarz, Stefan
Braun, Sascha D.
Monecke, Stefan
Ehricht, Ralf
Korus, Maciej
Benković, Damir
Korzeniowska, Malgorzata
Loncaric, Igor
The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title_full The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title_fullStr The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title_full_unstemmed The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title_short The First Report of mcr-1-Carrying Escherichia coli Originating from Animals in Serbia
title_sort first report of mcr-1-carrying escherichia coli originating from animals in serbia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467794/
https://www.ncbi.nlm.nih.gov/pubmed/34572647
http://dx.doi.org/10.3390/antibiotics10091063
work_keys_str_mv AT misicdusan thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT kiskarolyferenc thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT szostakmichaelp thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT cabaladriana thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT ruppitschwerner thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT bernreiterhofertanja thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT milovanovicviktoria thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT feßlerandreat thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT allerbergerfranz thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT spergserjoachim thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT mullerelke thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT schwarzstefan thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT braunsaschad thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT moneckestefan thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT ehrichtralf thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT korusmaciej thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT benkovicdamir thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT korzeniowskamalgorzata thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT loncaricigor thefirstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT misicdusan firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT kiskarolyferenc firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT szostakmichaelp firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT cabaladriana firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT ruppitschwerner firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT bernreiterhofertanja firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT milovanovicviktoria firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT feßlerandreat firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT allerbergerfranz firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT spergserjoachim firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT mullerelke firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT schwarzstefan firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT braunsaschad firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT moneckestefan firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT ehrichtralf firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT korusmaciej firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT benkovicdamir firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT korzeniowskamalgorzata firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia
AT loncaricigor firstreportofmcr1carryingescherichiacolioriginatingfromanimalsinserbia