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Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study

Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. However, whether these metabolites play an active role in pathogenesis of hypertension or are altered because of this has yet to be determined. In...

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Autores principales: Baranwal, Gaurav, Pilla, Rachel, Goodlett, Bethany L., Coleman, Aja K., Arenaz, Cristina M., Jayaraman, Arul, Rutkowski, Joseph M., Alaniz, Robert C., Mitchell, Brett M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467937/
https://www.ncbi.nlm.nih.gov/pubmed/34572600
http://dx.doi.org/10.3390/biom11091387
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author Baranwal, Gaurav
Pilla, Rachel
Goodlett, Bethany L.
Coleman, Aja K.
Arenaz, Cristina M.
Jayaraman, Arul
Rutkowski, Joseph M.
Alaniz, Robert C.
Mitchell, Brett M.
author_facet Baranwal, Gaurav
Pilla, Rachel
Goodlett, Bethany L.
Coleman, Aja K.
Arenaz, Cristina M.
Jayaraman, Arul
Rutkowski, Joseph M.
Alaniz, Robert C.
Mitchell, Brett M.
author_sort Baranwal, Gaurav
collection PubMed
description Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. However, whether these metabolites play an active role in pathogenesis of hypertension or are altered because of this has yet to be determined. In the current study, we hypothesized that metabolite changes common between hypertension models may unify hypertension’s pathophysiology with respect to metabolites. We utilized two common mouse models of experimental hypertension: L-arginine methyl ester hydrochloride (L-NAME)/high-salt-diet-induced hypertension (LSHTN) and angiotensin II induced hypertension (AHTN). To identify common metabolites that were altered across both models, we performed untargeted global metabolomics analysis in serum and urine and the resulting data were analyzed using MetaboAnalyst software and compared to control mice. A total of 41 serum metabolites were identified as being significantly altered in any hypertensive model compared to the controls. Of these compounds, 14 were commonly changed in both hypertensive groups, with 4 significantly increased and 10 significantly decreased. In the urine, six metabolites were significantly altered in any hypertensive group with respect to the control; however, none of them were common between the hypertensive groups. These findings demonstrate that a modest, but potentially important, number of serum metabolites are commonly altered between experimental hypertension models. Further studies of the newly identified metabolites from this untargeted metabolomics analysis may lead to a greater understanding of the association between gut dysbiosis and hypertension.
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spelling pubmed-84679372021-09-27 Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study Baranwal, Gaurav Pilla, Rachel Goodlett, Bethany L. Coleman, Aja K. Arenaz, Cristina M. Jayaraman, Arul Rutkowski, Joseph M. Alaniz, Robert C. Mitchell, Brett M. Biomolecules Article Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. However, whether these metabolites play an active role in pathogenesis of hypertension or are altered because of this has yet to be determined. In the current study, we hypothesized that metabolite changes common between hypertension models may unify hypertension’s pathophysiology with respect to metabolites. We utilized two common mouse models of experimental hypertension: L-arginine methyl ester hydrochloride (L-NAME)/high-salt-diet-induced hypertension (LSHTN) and angiotensin II induced hypertension (AHTN). To identify common metabolites that were altered across both models, we performed untargeted global metabolomics analysis in serum and urine and the resulting data were analyzed using MetaboAnalyst software and compared to control mice. A total of 41 serum metabolites were identified as being significantly altered in any hypertensive model compared to the controls. Of these compounds, 14 were commonly changed in both hypertensive groups, with 4 significantly increased and 10 significantly decreased. In the urine, six metabolites were significantly altered in any hypertensive group with respect to the control; however, none of them were common between the hypertensive groups. These findings demonstrate that a modest, but potentially important, number of serum metabolites are commonly altered between experimental hypertension models. Further studies of the newly identified metabolites from this untargeted metabolomics analysis may lead to a greater understanding of the association between gut dysbiosis and hypertension. MDPI 2021-09-21 /pmc/articles/PMC8467937/ /pubmed/34572600 http://dx.doi.org/10.3390/biom11091387 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baranwal, Gaurav
Pilla, Rachel
Goodlett, Bethany L.
Coleman, Aja K.
Arenaz, Cristina M.
Jayaraman, Arul
Rutkowski, Joseph M.
Alaniz, Robert C.
Mitchell, Brett M.
Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title_full Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title_fullStr Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title_full_unstemmed Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title_short Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study
title_sort common metabolites in two different hypertensive mouse models: a serum and urine metabolome study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467937/
https://www.ncbi.nlm.nih.gov/pubmed/34572600
http://dx.doi.org/10.3390/biom11091387
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