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Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection

Fusarium species are common plant pathogens that cause several important diseases. They produce a wide range of secondary metabolites, among which mycotoxins and extracellular cell wall-degrading enzymes (CWDEs) contribute to weakening and invading the host plant successfully. Two species of Fusariu...

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Autores principales: Perincherry, Lakshmipriya, Urbaniak, Monika, Pawłowicz, Izabela, Kotowska, Karolina, Waśkiewicz, Agnieszka, Stępień, Łukasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467997/
https://www.ncbi.nlm.nih.gov/pubmed/34576051
http://dx.doi.org/10.3390/ijms22189888
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author Perincherry, Lakshmipriya
Urbaniak, Monika
Pawłowicz, Izabela
Kotowska, Karolina
Waśkiewicz, Agnieszka
Stępień, Łukasz
author_facet Perincherry, Lakshmipriya
Urbaniak, Monika
Pawłowicz, Izabela
Kotowska, Karolina
Waśkiewicz, Agnieszka
Stępień, Łukasz
author_sort Perincherry, Lakshmipriya
collection PubMed
description Fusarium species are common plant pathogens that cause several important diseases. They produce a wide range of secondary metabolites, among which mycotoxins and extracellular cell wall-degrading enzymes (CWDEs) contribute to weakening and invading the host plant successfully. Two species of Fusarium isolated from peas were monitored for their expression profile of three cell wall-degrading enzyme coding genes upon culturing with extracts from resistant (Sokolik) and susceptible (Santana) pea cultivars. The extracts from Santana induced a sudden increase in the gene expression, whereas Sokolik elicited a reduced expression. The coherent observation was that the biochemical profile of the host plant plays a major role in regulating the fungal gene expression. In order to uncover the fungal characteristics in planta, both pea cultivars were infected with two strains each of F. proliferatum and F. oxysporum on the 30th day of growth. The enzyme activity assays from both roots and rhizosphere indicated that more enzymes were used for degrading the cell wall of the resistant host compared to the susceptible host. The most commonly produced enzymes were cellulase, β-glucosidase, xylanase, pectinase and lipase, where the pathogen selectively degraded the components of both the primary and secondary cell walls. The levels of beauvericin accumulated in the infected roots of both cultivars were also monitored. There was a difference between the levels of beauvericin accumulated in both the cultivars, where the susceptible cultivar had more beauvericin than the resistant one, showing that the plants susceptible to the pathogen were also susceptible to the toxin accumulation.
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spelling pubmed-84679972021-09-27 Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection Perincherry, Lakshmipriya Urbaniak, Monika Pawłowicz, Izabela Kotowska, Karolina Waśkiewicz, Agnieszka Stępień, Łukasz Int J Mol Sci Article Fusarium species are common plant pathogens that cause several important diseases. They produce a wide range of secondary metabolites, among which mycotoxins and extracellular cell wall-degrading enzymes (CWDEs) contribute to weakening and invading the host plant successfully. Two species of Fusarium isolated from peas were monitored for their expression profile of three cell wall-degrading enzyme coding genes upon culturing with extracts from resistant (Sokolik) and susceptible (Santana) pea cultivars. The extracts from Santana induced a sudden increase in the gene expression, whereas Sokolik elicited a reduced expression. The coherent observation was that the biochemical profile of the host plant plays a major role in regulating the fungal gene expression. In order to uncover the fungal characteristics in planta, both pea cultivars were infected with two strains each of F. proliferatum and F. oxysporum on the 30th day of growth. The enzyme activity assays from both roots and rhizosphere indicated that more enzymes were used for degrading the cell wall of the resistant host compared to the susceptible host. The most commonly produced enzymes were cellulase, β-glucosidase, xylanase, pectinase and lipase, where the pathogen selectively degraded the components of both the primary and secondary cell walls. The levels of beauvericin accumulated in the infected roots of both cultivars were also monitored. There was a difference between the levels of beauvericin accumulated in both the cultivars, where the susceptible cultivar had more beauvericin than the resistant one, showing that the plants susceptible to the pathogen were also susceptible to the toxin accumulation. MDPI 2021-09-13 /pmc/articles/PMC8467997/ /pubmed/34576051 http://dx.doi.org/10.3390/ijms22189888 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perincherry, Lakshmipriya
Urbaniak, Monika
Pawłowicz, Izabela
Kotowska, Karolina
Waśkiewicz, Agnieszka
Stępień, Łukasz
Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title_full Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title_fullStr Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title_full_unstemmed Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title_short Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants’ Infection
title_sort dynamics of fusarium mycotoxins and lytic enzymes during pea plants’ infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467997/
https://www.ncbi.nlm.nih.gov/pubmed/34576051
http://dx.doi.org/10.3390/ijms22189888
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