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Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging
Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a hig...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468006/ https://www.ncbi.nlm.nih.gov/pubmed/34562919 http://dx.doi.org/10.3390/bios11090330 |
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author | Zhang, Jia Gao, Xinwei Wang, Luwei Guo, Yong Zhu, Yinru Yang, Zhigang Yan, Wei Qu, Junle |
author_facet | Zhang, Jia Gao, Xinwei Wang, Luwei Guo, Yong Zhu, Yinru Yang, Zhigang Yan, Wei Qu, Junle |
author_sort | Zhang, Jia |
collection | PubMed |
description | Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells. |
format | Online Article Text |
id | pubmed-8468006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84680062021-09-27 Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging Zhang, Jia Gao, Xinwei Wang, Luwei Guo, Yong Zhu, Yinru Yang, Zhigang Yan, Wei Qu, Junle Biosensors (Basel) Article Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells. MDPI 2021-09-10 /pmc/articles/PMC8468006/ /pubmed/34562919 http://dx.doi.org/10.3390/bios11090330 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Jia Gao, Xinwei Wang, Luwei Guo, Yong Zhu, Yinru Yang, Zhigang Yan, Wei Qu, Junle Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title | Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title_full | Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title_fullStr | Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title_full_unstemmed | Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title_short | Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging |
title_sort | low-power two-color stimulated emission depletion microscopy for live cell imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468006/ https://www.ncbi.nlm.nih.gov/pubmed/34562919 http://dx.doi.org/10.3390/bios11090330 |
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