Cargando…

Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes

Aspens (Populus tremula and its hybrids), economically and ecologically important fast-growing trees, are often damaged by Phellinus tremulae, a rot-causing fungus. Plant-associated bacteria can be used to increase plant growth and resistance; however, no systematic studies relating the activity of...

Descripción completa

Detalles Bibliográficos
Autores principales: Striganavičiūtė, Greta, Žiauka, Jonas, Sirgedaitė-Šėžienė, Vaida, Vaitiekūnaitė, Dorotėja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468027/
https://www.ncbi.nlm.nih.gov/pubmed/34576797
http://dx.doi.org/10.3390/microorganisms9091901
_version_ 1784573555091963904
author Striganavičiūtė, Greta
Žiauka, Jonas
Sirgedaitė-Šėžienė, Vaida
Vaitiekūnaitė, Dorotėja
author_facet Striganavičiūtė, Greta
Žiauka, Jonas
Sirgedaitė-Šėžienė, Vaida
Vaitiekūnaitė, Dorotėja
author_sort Striganavičiūtė, Greta
collection PubMed
description Aspens (Populus tremula and its hybrids), economically and ecologically important fast-growing trees, are often damaged by Phellinus tremulae, a rot-causing fungus. Plant-associated bacteria can be used to increase plant growth and resistance; however, no systematic studies relating the activity of symbiotic bacteria to aspen resistance against Phellinus tremulae have been conducted so far. The present pioneer study investigated the responses of two Populus tremula and two P. tremula × P. tremuloides genotypes to in vitro inoculations with, first, either Pseudomonas sp. or Paenibacillus sp. bacteria (isolated originally from hybrid aspen tissue cultures and being most closely related to Pseudomonas oryzihabitans and Paenibacillus tundrae, respectively) and, in the subsequent stage, with Phellinus tremulae. Both morphological parameters of in vitro-grown plants and biochemical content of their leaves, including photosynthesis pigments and secondary metabolites, were analyzed. It was found that both Populus tremula × P. tremuloides genotypes, whose development in vitro was significantly damaged by Phellinus tremulae, were characterized by certain responses to the studied bacteria: decreased shoot development by both Paenibacillus sp. and Pseudomonas sp. and increased phenol content by Pseudomonas sp. In turn, these responses were lacking in both Populus tremula genotypes that showed in vitro resistance to the fungus. Moreover, these genotypes showed positive long-term growth responses to bacterial inoculation, even synergistic with the subsequent fungal inoculation. Hence, the studied bacteria were demonstrated as a potential tool for the improved in vitro propagation of fungus-resistant aspen genotypes.
format Online
Article
Text
id pubmed-8468027
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84680272021-09-27 Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes Striganavičiūtė, Greta Žiauka, Jonas Sirgedaitė-Šėžienė, Vaida Vaitiekūnaitė, Dorotėja Microorganisms Article Aspens (Populus tremula and its hybrids), economically and ecologically important fast-growing trees, are often damaged by Phellinus tremulae, a rot-causing fungus. Plant-associated bacteria can be used to increase plant growth and resistance; however, no systematic studies relating the activity of symbiotic bacteria to aspen resistance against Phellinus tremulae have been conducted so far. The present pioneer study investigated the responses of two Populus tremula and two P. tremula × P. tremuloides genotypes to in vitro inoculations with, first, either Pseudomonas sp. or Paenibacillus sp. bacteria (isolated originally from hybrid aspen tissue cultures and being most closely related to Pseudomonas oryzihabitans and Paenibacillus tundrae, respectively) and, in the subsequent stage, with Phellinus tremulae. Both morphological parameters of in vitro-grown plants and biochemical content of their leaves, including photosynthesis pigments and secondary metabolites, were analyzed. It was found that both Populus tremula × P. tremuloides genotypes, whose development in vitro was significantly damaged by Phellinus tremulae, were characterized by certain responses to the studied bacteria: decreased shoot development by both Paenibacillus sp. and Pseudomonas sp. and increased phenol content by Pseudomonas sp. In turn, these responses were lacking in both Populus tremula genotypes that showed in vitro resistance to the fungus. Moreover, these genotypes showed positive long-term growth responses to bacterial inoculation, even synergistic with the subsequent fungal inoculation. Hence, the studied bacteria were demonstrated as a potential tool for the improved in vitro propagation of fungus-resistant aspen genotypes. MDPI 2021-09-07 /pmc/articles/PMC8468027/ /pubmed/34576797 http://dx.doi.org/10.3390/microorganisms9091901 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Striganavičiūtė, Greta
Žiauka, Jonas
Sirgedaitė-Šėžienė, Vaida
Vaitiekūnaitė, Dorotėja
Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title_full Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title_fullStr Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title_full_unstemmed Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title_short Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes
title_sort impact of plant-associated bacteria on the in vitro growth and pathogenic resistance against phellinus tremulae of different aspen (populus) genotypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468027/
https://www.ncbi.nlm.nih.gov/pubmed/34576797
http://dx.doi.org/10.3390/microorganisms9091901
work_keys_str_mv AT striganaviciutegreta impactofplantassociatedbacteriaontheinvitrogrowthandpathogenicresistanceagainstphellinustremulaeofdifferentaspenpopulusgenotypes
AT ziaukajonas impactofplantassociatedbacteriaontheinvitrogrowthandpathogenicresistanceagainstphellinustremulaeofdifferentaspenpopulusgenotypes
AT sirgedaitesezienevaida impactofplantassociatedbacteriaontheinvitrogrowthandpathogenicresistanceagainstphellinustremulaeofdifferentaspenpopulusgenotypes
AT vaitiekunaitedoroteja impactofplantassociatedbacteriaontheinvitrogrowthandpathogenicresistanceagainstphellinustremulaeofdifferentaspenpopulusgenotypes