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Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach
Poloxamer188 (PL188), as one of the most commonly used pharmaceutical excipients, has unique physicochemical properties and good biocompatibility, and so is playing an increasingly extensive role in the field of medicine. Currently, there are few studies on the tissue distribution of PL188 in vivo....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468058/ https://www.ncbi.nlm.nih.gov/pubmed/34577115 http://dx.doi.org/10.3390/molecules26185644 |
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author | Feng, Yixuan Li, Lele Li, Yuxuan Zhou, Xinxin Lin, Xiaoying Cui, Yue Zhu, Heyun Feng, Bo |
author_facet | Feng, Yixuan Li, Lele Li, Yuxuan Zhou, Xinxin Lin, Xiaoying Cui, Yue Zhu, Heyun Feng, Bo |
author_sort | Feng, Yixuan |
collection | PubMed |
description | Poloxamer188 (PL188), as one of the most commonly used pharmaceutical excipients, has unique physicochemical properties and good biocompatibility, and so is playing an increasingly extensive role in the field of medicine. Currently, there are few studies on the tissue distribution of PL188 in vivo. In this study, the LC-MS method based on MS(ALL) technique of quadrupole time of flight mass spectrometry for absolute quantitative analysis of poloxamer 188 in biological substrates was established for the first time. The tissue distribution of poloxamer188 in SD rats were studied using the established quantitative analysis method. To explore the distribution of PL188 in organs and tissues, PL188 was administered via rat tail vein at a dose of 5 mg/kg. Eight kinds of tissues including heart, liver, spleen, lung, kidney, stomach, muscle and brain of rats were collected at 0.25 h, 1 h and 4 h after administration. Tissue distributions showed the highest level was observed in kidney, then in stomach, which indicated PL188 mainly bioaccumulated in the kidney. This study can provide references for the further study of PL188. |
format | Online Article Text |
id | pubmed-8468058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84680582021-09-27 Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach Feng, Yixuan Li, Lele Li, Yuxuan Zhou, Xinxin Lin, Xiaoying Cui, Yue Zhu, Heyun Feng, Bo Molecules Article Poloxamer188 (PL188), as one of the most commonly used pharmaceutical excipients, has unique physicochemical properties and good biocompatibility, and so is playing an increasingly extensive role in the field of medicine. Currently, there are few studies on the tissue distribution of PL188 in vivo. In this study, the LC-MS method based on MS(ALL) technique of quadrupole time of flight mass spectrometry for absolute quantitative analysis of poloxamer 188 in biological substrates was established for the first time. The tissue distribution of poloxamer188 in SD rats were studied using the established quantitative analysis method. To explore the distribution of PL188 in organs and tissues, PL188 was administered via rat tail vein at a dose of 5 mg/kg. Eight kinds of tissues including heart, liver, spleen, lung, kidney, stomach, muscle and brain of rats were collected at 0.25 h, 1 h and 4 h after administration. Tissue distributions showed the highest level was observed in kidney, then in stomach, which indicated PL188 mainly bioaccumulated in the kidney. This study can provide references for the further study of PL188. MDPI 2021-09-17 /pmc/articles/PMC8468058/ /pubmed/34577115 http://dx.doi.org/10.3390/molecules26185644 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feng, Yixuan Li, Lele Li, Yuxuan Zhou, Xinxin Lin, Xiaoying Cui, Yue Zhu, Heyun Feng, Bo Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title | Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title_full | Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title_fullStr | Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title_full_unstemmed | Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title_short | Tissue Distribution Study of Poloxamer188 in Rats by Ultra-High-Performance Liquid Chromatography Quadrupole Time of Flight/Mass Spectrometry with MS(ALL)-Based Approach |
title_sort | tissue distribution study of poloxamer188 in rats by ultra-high-performance liquid chromatography quadrupole time of flight/mass spectrometry with ms(all)-based approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468058/ https://www.ncbi.nlm.nih.gov/pubmed/34577115 http://dx.doi.org/10.3390/molecules26185644 |
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