Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli

Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding...

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Autores principales: Yeom, Chang-Hun, Jeong, Hee-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468072/
https://www.ncbi.nlm.nih.gov/pubmed/34577904
http://dx.doi.org/10.3390/medicina57090981
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author Yeom, Chang-Hun
Jeong, Hee-Jin
author_facet Yeom, Chang-Hun
Jeong, Hee-Jin
author_sort Yeom, Chang-Hun
collection PubMed
description Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases.
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spelling pubmed-84680722021-09-27 Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli Yeom, Chang-Hun Jeong, Hee-Jin Medicina (Kaunas) Communication Matrix metalloproteinase 9 (MMP9) is involved in several aspects of the pathology of cancer, including invasion, metastasis, and angiogenesis. In this study, we expressed a recombinant scFv-type anti-MMP9 antibody in soluble form using Escherichia coli, purified it, and confirmed its antigen-binding ability. The convenient, rapid, inexpressive system used in this study for producing recombinant antibody fragments needs only five days, and thus can be used for the efficient production of scFv against MMP9, which can be used in a range of applications and industrial fields, including diagnosis and treatment of inflammatory and cancer-related diseases. MDPI 2021-09-18 /pmc/articles/PMC8468072/ /pubmed/34577904 http://dx.doi.org/10.3390/medicina57090981 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yeom, Chang-Hun
Jeong, Hee-Jin
Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title_full Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title_fullStr Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title_full_unstemmed Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title_short Production of a Soluble Recombinant Antibody Fragment against MMP9 Using Escherichia coli
title_sort production of a soluble recombinant antibody fragment against mmp9 using escherichia coli
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468072/
https://www.ncbi.nlm.nih.gov/pubmed/34577904
http://dx.doi.org/10.3390/medicina57090981
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