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Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin

We have previously shown that two enteral nutrition formulas suppressed gastric lesions induced by the oral administration of indomethacin (IND) in mice. However, the mechanism of their protective effect is unknown. In this study, the effect of the two enteral nutrition formulas on gastric lesions i...

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Autores principales: Yamagishi, Yoshiaki, Saiki, Rei, Yoshimi, Takeshi, Kudo, Toshiyuki, Ito, Kiyomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468157/
https://www.ncbi.nlm.nih.gov/pubmed/34579173
http://dx.doi.org/10.3390/nu13093297
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author Yamagishi, Yoshiaki
Saiki, Rei
Yoshimi, Takeshi
Kudo, Toshiyuki
Ito, Kiyomi
author_facet Yamagishi, Yoshiaki
Saiki, Rei
Yoshimi, Takeshi
Kudo, Toshiyuki
Ito, Kiyomi
author_sort Yamagishi, Yoshiaki
collection PubMed
description We have previously shown that two enteral nutrition formulas suppressed gastric lesions induced by the oral administration of indomethacin (IND) in mice. However, the mechanism of their protective effect is unknown. In this study, the effect of the two enteral nutrition formulas on gastric lesions induced by subcutaneous IND injection was investigated, with the objective of exploring the possibility that they may interact directly with IND in the gastrointestinal tract. Ten-week-old, male, ICR mice were fasted, then orally given either purified water, Mermed(®) One, or 2-fold diluted Terumeal(®) 2.0α as enteral nutrition formula (25 mL/kg). IND was injected subcutaneously at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of mice given enteral nutrition formula was reduced to 56–89% and 34–61%, respectively, compared with the mice given purified water. The time courses of plasma IND concentrations were comparable among the three groups. These results suggested that the effect of these enteral nutrition formulas on gastric lesions did not originate from their direct interaction with IND in the gastrointestinal tract or their effect on the disposition of IND.
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spelling pubmed-84681572021-09-27 Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin Yamagishi, Yoshiaki Saiki, Rei Yoshimi, Takeshi Kudo, Toshiyuki Ito, Kiyomi Nutrients Article We have previously shown that two enteral nutrition formulas suppressed gastric lesions induced by the oral administration of indomethacin (IND) in mice. However, the mechanism of their protective effect is unknown. In this study, the effect of the two enteral nutrition formulas on gastric lesions induced by subcutaneous IND injection was investigated, with the objective of exploring the possibility that they may interact directly with IND in the gastrointestinal tract. Ten-week-old, male, ICR mice were fasted, then orally given either purified water, Mermed(®) One, or 2-fold diluted Terumeal(®) 2.0α as enteral nutrition formula (25 mL/kg). IND was injected subcutaneously at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of mice given enteral nutrition formula was reduced to 56–89% and 34–61%, respectively, compared with the mice given purified water. The time courses of plasma IND concentrations were comparable among the three groups. These results suggested that the effect of these enteral nutrition formulas on gastric lesions did not originate from their direct interaction with IND in the gastrointestinal tract or their effect on the disposition of IND. MDPI 2021-09-21 /pmc/articles/PMC8468157/ /pubmed/34579173 http://dx.doi.org/10.3390/nu13093297 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamagishi, Yoshiaki
Saiki, Rei
Yoshimi, Takeshi
Kudo, Toshiyuki
Ito, Kiyomi
Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title_full Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title_fullStr Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title_full_unstemmed Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title_short Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin
title_sort gastroprotective effect of enteral nutrition formula in mice injected subcutaneously with indomethacin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468157/
https://www.ncbi.nlm.nih.gov/pubmed/34579173
http://dx.doi.org/10.3390/nu13093297
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