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A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach
Bacteriophages can be used in various applications, from the classical approach as substitutes for antibiotics (phage therapy) to new biotechnological uses, i.e., as a protein delivery vehicle, a diagnostic tool for specific strains of bacteria (phage typing), or environmental bioremediation. The de...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468216/ https://www.ncbi.nlm.nih.gov/pubmed/34578135 http://dx.doi.org/10.3390/pathogens10091100 |
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author | Silva, Jessica Dias, Roberto Junior, José Ivo Marcelino, Maraísa Silva, Mirelly Carmo, Adriele Sousa, Maira Silva, Cynthia de Paula, Sergio |
author_facet | Silva, Jessica Dias, Roberto Junior, José Ivo Marcelino, Maraísa Silva, Mirelly Carmo, Adriele Sousa, Maira Silva, Cynthia de Paula, Sergio |
author_sort | Silva, Jessica |
collection | PubMed |
description | Bacteriophages can be used in various applications, from the classical approach as substitutes for antibiotics (phage therapy) to new biotechnological uses, i.e., as a protein delivery vehicle, a diagnostic tool for specific strains of bacteria (phage typing), or environmental bioremediation. The demand for bacteriophage production increases daily, and studies that improve these production processes are necessary. This study evaluated the production of a T4-like bacteriophage vB_EcoM-UFV09 (an E. coli-infecting phage with high potential for reducing environmental biofilms) in seven types of culture media (Luria–Bertani broth and the M9 minimal medium with six different carbon sources) employing four cultivation variables (temperature, incubation time, agitation, and multiplicity of infection). For this purpose, the rotatable central composite design (RCCD) methodology was used, combining and comparing all parameters to determine the ideal conditions for starting to scale up the production process. We used the RCCD to set up the experimental design by combining the cultivation parameters in a specific and systematic way. Despite the high number of conditions evaluated, the results showed that when specific conditions were utilized, viral production was effective even when using a minimal medium, such as M9/glucose, which is less expensive and can significantly reduce costs during large-scale phage production. |
format | Online Article Text |
id | pubmed-8468216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84682162021-09-27 A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach Silva, Jessica Dias, Roberto Junior, José Ivo Marcelino, Maraísa Silva, Mirelly Carmo, Adriele Sousa, Maira Silva, Cynthia de Paula, Sergio Pathogens Article Bacteriophages can be used in various applications, from the classical approach as substitutes for antibiotics (phage therapy) to new biotechnological uses, i.e., as a protein delivery vehicle, a diagnostic tool for specific strains of bacteria (phage typing), or environmental bioremediation. The demand for bacteriophage production increases daily, and studies that improve these production processes are necessary. This study evaluated the production of a T4-like bacteriophage vB_EcoM-UFV09 (an E. coli-infecting phage with high potential for reducing environmental biofilms) in seven types of culture media (Luria–Bertani broth and the M9 minimal medium with six different carbon sources) employing four cultivation variables (temperature, incubation time, agitation, and multiplicity of infection). For this purpose, the rotatable central composite design (RCCD) methodology was used, combining and comparing all parameters to determine the ideal conditions for starting to scale up the production process. We used the RCCD to set up the experimental design by combining the cultivation parameters in a specific and systematic way. Despite the high number of conditions evaluated, the results showed that when specific conditions were utilized, viral production was effective even when using a minimal medium, such as M9/glucose, which is less expensive and can significantly reduce costs during large-scale phage production. MDPI 2021-08-29 /pmc/articles/PMC8468216/ /pubmed/34578135 http://dx.doi.org/10.3390/pathogens10091100 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silva, Jessica Dias, Roberto Junior, José Ivo Marcelino, Maraísa Silva, Mirelly Carmo, Adriele Sousa, Maira Silva, Cynthia de Paula, Sergio A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title | A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title_full | A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title_fullStr | A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title_full_unstemmed | A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title_short | A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach |
title_sort | rapid method for performing a multivariate optimization of phage production using the rccd approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468216/ https://www.ncbi.nlm.nih.gov/pubmed/34578135 http://dx.doi.org/10.3390/pathogens10091100 |
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