Cargando…
Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too o...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468251/ https://www.ncbi.nlm.nih.gov/pubmed/34577794 http://dx.doi.org/10.3390/medicina57090871 |
_version_ | 1784573617422467072 |
---|---|
author | Makiala-Mandanda, Sheila Abbate, Jessica L. Pukuta-Simbu, Elisabeth Ahuka-Mundeke, Steve Muyembe-Tamfum, Jean-Jacques Leroy, Eric M. Becquart, Pierre |
author_facet | Makiala-Mandanda, Sheila Abbate, Jessica L. Pukuta-Simbu, Elisabeth Ahuka-Mundeke, Steve Muyembe-Tamfum, Jean-Jacques Leroy, Eric M. Becquart, Pierre |
author_sort | Makiala-Mandanda, Sheila |
collection | PubMed |
description | In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too often left without a diagnosis. By retroactively screening samples for other potential viral infections, we can both try to find sources of patient disease and gain information on how commonly they may occur and co-occur. Several human arboviruses have previously been identified, but there remain many other viral families that could be responsible for acute febrile jaundice. Here, we assessed the prevalence of human herpes viruses (HHVs) in these acute febrile jaundice disease samples. Total viral DNA was extracted from serum of 451 patients with acute febrile jaundice. We used real-time quantitative PCR to test all specimens for cytomegalovirus (CMV), herpes simplex virus (HSV), human herpes virus type 6 (HHV-6) and varicella-zoster virus (VZV). We found 21.3% had active HHV replication (13.1%, 2.4%, 6.2% and 2.4% were positive for CMV, HSV, HHV-6 and VZV, respectively), and that nearly half (45.8%) of these infections were characterized by co-infection either among HHVs or between HHVs and other viral infection, sometimes associated with acute febrile jaundice previously identified. Our results show that the role of HHV primary infection or reactivation in contributing to acute febrile jaundice disease identified through the yellow fever surveillance program should be routinely considered in diagnosing these patients. |
format | Online Article Text |
id | pubmed-8468251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84682512021-09-27 Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo Makiala-Mandanda, Sheila Abbate, Jessica L. Pukuta-Simbu, Elisabeth Ahuka-Mundeke, Steve Muyembe-Tamfum, Jean-Jacques Leroy, Eric M. Becquart, Pierre Medicina (Kaunas) Brief Report In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too often left without a diagnosis. By retroactively screening samples for other potential viral infections, we can both try to find sources of patient disease and gain information on how commonly they may occur and co-occur. Several human arboviruses have previously been identified, but there remain many other viral families that could be responsible for acute febrile jaundice. Here, we assessed the prevalence of human herpes viruses (HHVs) in these acute febrile jaundice disease samples. Total viral DNA was extracted from serum of 451 patients with acute febrile jaundice. We used real-time quantitative PCR to test all specimens for cytomegalovirus (CMV), herpes simplex virus (HSV), human herpes virus type 6 (HHV-6) and varicella-zoster virus (VZV). We found 21.3% had active HHV replication (13.1%, 2.4%, 6.2% and 2.4% were positive for CMV, HSV, HHV-6 and VZV, respectively), and that nearly half (45.8%) of these infections were characterized by co-infection either among HHVs or between HHVs and other viral infection, sometimes associated with acute febrile jaundice previously identified. Our results show that the role of HHV primary infection or reactivation in contributing to acute febrile jaundice disease identified through the yellow fever surveillance program should be routinely considered in diagnosing these patients. MDPI 2021-08-25 /pmc/articles/PMC8468251/ /pubmed/34577794 http://dx.doi.org/10.3390/medicina57090871 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Makiala-Mandanda, Sheila Abbate, Jessica L. Pukuta-Simbu, Elisabeth Ahuka-Mundeke, Steve Muyembe-Tamfum, Jean-Jacques Leroy, Eric M. Becquart, Pierre Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title | Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title_full | Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title_fullStr | Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title_full_unstemmed | Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title_short | Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo |
title_sort | herpes infections in suspected cases of yellow fever in the democratic republic of the congo |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468251/ https://www.ncbi.nlm.nih.gov/pubmed/34577794 http://dx.doi.org/10.3390/medicina57090871 |
work_keys_str_mv | AT makialamandandasheila herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT abbatejessical herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT pukutasimbuelisabeth herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT ahukamundekesteve herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT muyembetamfumjeanjacques herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT leroyericm herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo AT becquartpierre herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo |