Cargando…

Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo

In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too o...

Descripción completa

Detalles Bibliográficos
Autores principales: Makiala-Mandanda, Sheila, Abbate, Jessica L., Pukuta-Simbu, Elisabeth, Ahuka-Mundeke, Steve, Muyembe-Tamfum, Jean-Jacques, Leroy, Eric M., Becquart, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468251/
https://www.ncbi.nlm.nih.gov/pubmed/34577794
http://dx.doi.org/10.3390/medicina57090871
_version_ 1784573617422467072
author Makiala-Mandanda, Sheila
Abbate, Jessica L.
Pukuta-Simbu, Elisabeth
Ahuka-Mundeke, Steve
Muyembe-Tamfum, Jean-Jacques
Leroy, Eric M.
Becquart, Pierre
author_facet Makiala-Mandanda, Sheila
Abbate, Jessica L.
Pukuta-Simbu, Elisabeth
Ahuka-Mundeke, Steve
Muyembe-Tamfum, Jean-Jacques
Leroy, Eric M.
Becquart, Pierre
author_sort Makiala-Mandanda, Sheila
collection PubMed
description In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too often left without a diagnosis. By retroactively screening samples for other potential viral infections, we can both try to find sources of patient disease and gain information on how commonly they may occur and co-occur. Several human arboviruses have previously been identified, but there remain many other viral families that could be responsible for acute febrile jaundice. Here, we assessed the prevalence of human herpes viruses (HHVs) in these acute febrile jaundice disease samples. Total viral DNA was extracted from serum of 451 patients with acute febrile jaundice. We used real-time quantitative PCR to test all specimens for cytomegalovirus (CMV), herpes simplex virus (HSV), human herpes virus type 6 (HHV-6) and varicella-zoster virus (VZV). We found 21.3% had active HHV replication (13.1%, 2.4%, 6.2% and 2.4% were positive for CMV, HSV, HHV-6 and VZV, respectively), and that nearly half (45.8%) of these infections were characterized by co-infection either among HHVs or between HHVs and other viral infection, sometimes associated with acute febrile jaundice previously identified. Our results show that the role of HHV primary infection or reactivation in contributing to acute febrile jaundice disease identified through the yellow fever surveillance program should be routinely considered in diagnosing these patients.
format Online
Article
Text
id pubmed-8468251
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84682512021-09-27 Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo Makiala-Mandanda, Sheila Abbate, Jessica L. Pukuta-Simbu, Elisabeth Ahuka-Mundeke, Steve Muyembe-Tamfum, Jean-Jacques Leroy, Eric M. Becquart, Pierre Medicina (Kaunas) Brief Report In the battle to quickly identify potential yellow fever arbovirus outbreaks in the Democratic Republic of the Congo, active syndromic surveillance of acute febrile jaundice patients across the country is a powerful tool. However, patients who test negative for yellow fever virus infection are too often left without a diagnosis. By retroactively screening samples for other potential viral infections, we can both try to find sources of patient disease and gain information on how commonly they may occur and co-occur. Several human arboviruses have previously been identified, but there remain many other viral families that could be responsible for acute febrile jaundice. Here, we assessed the prevalence of human herpes viruses (HHVs) in these acute febrile jaundice disease samples. Total viral DNA was extracted from serum of 451 patients with acute febrile jaundice. We used real-time quantitative PCR to test all specimens for cytomegalovirus (CMV), herpes simplex virus (HSV), human herpes virus type 6 (HHV-6) and varicella-zoster virus (VZV). We found 21.3% had active HHV replication (13.1%, 2.4%, 6.2% and 2.4% were positive for CMV, HSV, HHV-6 and VZV, respectively), and that nearly half (45.8%) of these infections were characterized by co-infection either among HHVs or between HHVs and other viral infection, sometimes associated with acute febrile jaundice previously identified. Our results show that the role of HHV primary infection or reactivation in contributing to acute febrile jaundice disease identified through the yellow fever surveillance program should be routinely considered in diagnosing these patients. MDPI 2021-08-25 /pmc/articles/PMC8468251/ /pubmed/34577794 http://dx.doi.org/10.3390/medicina57090871 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Makiala-Mandanda, Sheila
Abbate, Jessica L.
Pukuta-Simbu, Elisabeth
Ahuka-Mundeke, Steve
Muyembe-Tamfum, Jean-Jacques
Leroy, Eric M.
Becquart, Pierre
Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title_full Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title_fullStr Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title_full_unstemmed Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title_short Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo
title_sort herpes infections in suspected cases of yellow fever in the democratic republic of the congo
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468251/
https://www.ncbi.nlm.nih.gov/pubmed/34577794
http://dx.doi.org/10.3390/medicina57090871
work_keys_str_mv AT makialamandandasheila herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT abbatejessical herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT pukutasimbuelisabeth herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT ahukamundekesteve herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT muyembetamfumjeanjacques herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT leroyericm herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo
AT becquartpierre herpesinfectionsinsuspectedcasesofyellowfeverinthedemocraticrepublicofthecongo