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Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay

Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study expl...

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Autores principales: Ugrin, Milena, Dinic, Jelena, Jeremic, Sanja, Dragicevic, Sandra, Banovic Djeri, Bojana, Nikolic, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468300/
https://www.ncbi.nlm.nih.gov/pubmed/34578638
http://dx.doi.org/10.3390/nano11092322
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author Ugrin, Milena
Dinic, Jelena
Jeremic, Sanja
Dragicevic, Sandra
Banovic Djeri, Bojana
Nikolic, Aleksandra
author_facet Ugrin, Milena
Dinic, Jelena
Jeremic, Sanja
Dragicevic, Sandra
Banovic Djeri, Bojana
Nikolic, Aleksandra
author_sort Ugrin, Milena
collection PubMed
description Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study exploited excellent properties of the BNC to develop an efficient and low-cost in vitro cell migration assay. The BNC scaffold was introduced into a cell culture 24 h after the SW480 cells were seeded, and cells were allowed to enter the scaffold within the next 24–48 h. The cells were stained with different fluorophores either before or after the introduction of the scaffold in the culture. Untreated cells were observed to enter the BNC scaffold in significant numbers, form clusters and retain a high viability after 48 h. To validate the assay’s usability for drug development, the treatments of SW480 cells were performed using aspirin, an agent known to reduce the migratory potential of this cell line in culture. This study demonstrates the application of BNC as a scaffold for cell migration testing as a low-cost alternative to commercial assays based on the Boyden chamber principle. The assay could be further developed for routine use in cancer research and anticancer drug development.
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spelling pubmed-84683002021-09-27 Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay Ugrin, Milena Dinic, Jelena Jeremic, Sanja Dragicevic, Sandra Banovic Djeri, Bojana Nikolic, Aleksandra Nanomaterials (Basel) Article Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study exploited excellent properties of the BNC to develop an efficient and low-cost in vitro cell migration assay. The BNC scaffold was introduced into a cell culture 24 h after the SW480 cells were seeded, and cells were allowed to enter the scaffold within the next 24–48 h. The cells were stained with different fluorophores either before or after the introduction of the scaffold in the culture. Untreated cells were observed to enter the BNC scaffold in significant numbers, form clusters and retain a high viability after 48 h. To validate the assay’s usability for drug development, the treatments of SW480 cells were performed using aspirin, an agent known to reduce the migratory potential of this cell line in culture. This study demonstrates the application of BNC as a scaffold for cell migration testing as a low-cost alternative to commercial assays based on the Boyden chamber principle. The assay could be further developed for routine use in cancer research and anticancer drug development. MDPI 2021-09-07 /pmc/articles/PMC8468300/ /pubmed/34578638 http://dx.doi.org/10.3390/nano11092322 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ugrin, Milena
Dinic, Jelena
Jeremic, Sanja
Dragicevic, Sandra
Banovic Djeri, Bojana
Nikolic, Aleksandra
Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title_full Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title_fullStr Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title_full_unstemmed Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title_short Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
title_sort bacterial nanocellulose as a scaffold for in vitro cell migration assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468300/
https://www.ncbi.nlm.nih.gov/pubmed/34578638
http://dx.doi.org/10.3390/nano11092322
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