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Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach

Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (...

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Autores principales: Krobthong, Sucheewin, Yingchutrakul, Yodying, Visessanguan, Wonnop, Mahatnirunkul, Thanisorn, Samutrtai, Pawitrabhorn, Chaichana, Chartchai, Papan, Phakorn, Choowongkomon, Kiattawee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468392/
https://www.ncbi.nlm.nih.gov/pubmed/34574267
http://dx.doi.org/10.3390/foods10092157
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author Krobthong, Sucheewin
Yingchutrakul, Yodying
Visessanguan, Wonnop
Mahatnirunkul, Thanisorn
Samutrtai, Pawitrabhorn
Chaichana, Chartchai
Papan, Phakorn
Choowongkomon, Kiattawee
author_facet Krobthong, Sucheewin
Yingchutrakul, Yodying
Visessanguan, Wonnop
Mahatnirunkul, Thanisorn
Samutrtai, Pawitrabhorn
Chaichana, Chartchai
Papan, Phakorn
Choowongkomon, Kiattawee
author_sort Krobthong, Sucheewin
collection PubMed
description Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X(1)) and enzyme usage (X(2)) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X(1) at 8.63 h and X(2) at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity
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spelling pubmed-84683922021-09-27 Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach Krobthong, Sucheewin Yingchutrakul, Yodying Visessanguan, Wonnop Mahatnirunkul, Thanisorn Samutrtai, Pawitrabhorn Chaichana, Chartchai Papan, Phakorn Choowongkomon, Kiattawee Foods Article Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X(1)) and enzyme usage (X(2)) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X(1) at 8.63 h and X(2) at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity MDPI 2021-09-12 /pmc/articles/PMC8468392/ /pubmed/34574267 http://dx.doi.org/10.3390/foods10092157 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krobthong, Sucheewin
Yingchutrakul, Yodying
Visessanguan, Wonnop
Mahatnirunkul, Thanisorn
Samutrtai, Pawitrabhorn
Chaichana, Chartchai
Papan, Phakorn
Choowongkomon, Kiattawee
Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title_full Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title_fullStr Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title_full_unstemmed Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title_short Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
title_sort study of the lipolysis effect of nanoliposome-encapsulated ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468392/
https://www.ncbi.nlm.nih.gov/pubmed/34574267
http://dx.doi.org/10.3390/foods10092157
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