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Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach
Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468392/ https://www.ncbi.nlm.nih.gov/pubmed/34574267 http://dx.doi.org/10.3390/foods10092157 |
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author | Krobthong, Sucheewin Yingchutrakul, Yodying Visessanguan, Wonnop Mahatnirunkul, Thanisorn Samutrtai, Pawitrabhorn Chaichana, Chartchai Papan, Phakorn Choowongkomon, Kiattawee |
author_facet | Krobthong, Sucheewin Yingchutrakul, Yodying Visessanguan, Wonnop Mahatnirunkul, Thanisorn Samutrtai, Pawitrabhorn Chaichana, Chartchai Papan, Phakorn Choowongkomon, Kiattawee |
author_sort | Krobthong, Sucheewin |
collection | PubMed |
description | Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X(1)) and enzyme usage (X(2)) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X(1) at 8.63 h and X(2) at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity |
format | Online Article Text |
id | pubmed-8468392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84683922021-09-27 Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach Krobthong, Sucheewin Yingchutrakul, Yodying Visessanguan, Wonnop Mahatnirunkul, Thanisorn Samutrtai, Pawitrabhorn Chaichana, Chartchai Papan, Phakorn Choowongkomon, Kiattawee Foods Article Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X(1)) and enzyme usage (X(2)) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X(1) at 8.63 h and X(2) at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity MDPI 2021-09-12 /pmc/articles/PMC8468392/ /pubmed/34574267 http://dx.doi.org/10.3390/foods10092157 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Krobthong, Sucheewin Yingchutrakul, Yodying Visessanguan, Wonnop Mahatnirunkul, Thanisorn Samutrtai, Pawitrabhorn Chaichana, Chartchai Papan, Phakorn Choowongkomon, Kiattawee Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title | Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title_full | Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title_fullStr | Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title_full_unstemmed | Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title_short | Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach |
title_sort | study of the lipolysis effect of nanoliposome-encapsulated ganoderma lucidum protein hydrolysates on adipocyte cells using proteomics approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468392/ https://www.ncbi.nlm.nih.gov/pubmed/34574267 http://dx.doi.org/10.3390/foods10092157 |
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