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Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope

Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration in phosphate-b...

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Autores principales: Zoz, Fiona, Guyot, Stéphane, Grandvalet, Cosette, Ragon, Mélanie, Lesniewska, Eric, Dupont, Sébastien, Firmesse, Olivier, Carpentier, Brigitte, Beney, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468791/
https://www.ncbi.nlm.nih.gov/pubmed/34574112
http://dx.doi.org/10.3390/foods10092002
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author Zoz, Fiona
Guyot, Stéphane
Grandvalet, Cosette
Ragon, Mélanie
Lesniewska, Eric
Dupont, Sébastien
Firmesse, Olivier
Carpentier, Brigitte
Beney, Laurent
author_facet Zoz, Fiona
Guyot, Stéphane
Grandvalet, Cosette
Ragon, Mélanie
Lesniewska, Eric
Dupont, Sébastien
Firmesse, Olivier
Carpentier, Brigitte
Beney, Laurent
author_sort Zoz, Fiona
collection PubMed
description Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration in phosphate-buffered saline (PBS) at 75%, 68%, 43% and 11% RH on the bacterial envelope was investigated using flow cytometry and atomic force microscopy. Changes after rehydration in the protein secondary structure and peptidoglycan were investigated by infrared spectroscopy. Complementary cultivability measurements were performed by running dehydration–rehydration with combinations of NaCl (3–0.01%), distilled water, city water and PBS. The main results show that cell membrane permeability and cell envelope were greatly altered during dehydration in PBS at 68% RH followed by rapid rehydration. This damage led cells to recover only 67% of their initial volume after rehydration. Moreover, the most efficient way to destroy cells was dehydration and rehydration in city water. Our study indicates that rehydration of dried, sullied foods on surfaces may improve current cleaning procedures in the food industry.
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spelling pubmed-84687912021-09-27 Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope Zoz, Fiona Guyot, Stéphane Grandvalet, Cosette Ragon, Mélanie Lesniewska, Eric Dupont, Sébastien Firmesse, Olivier Carpentier, Brigitte Beney, Laurent Foods Article Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration in phosphate-buffered saline (PBS) at 75%, 68%, 43% and 11% RH on the bacterial envelope was investigated using flow cytometry and atomic force microscopy. Changes after rehydration in the protein secondary structure and peptidoglycan were investigated by infrared spectroscopy. Complementary cultivability measurements were performed by running dehydration–rehydration with combinations of NaCl (3–0.01%), distilled water, city water and PBS. The main results show that cell membrane permeability and cell envelope were greatly altered during dehydration in PBS at 68% RH followed by rapid rehydration. This damage led cells to recover only 67% of their initial volume after rehydration. Moreover, the most efficient way to destroy cells was dehydration and rehydration in city water. Our study indicates that rehydration of dried, sullied foods on surfaces may improve current cleaning procedures in the food industry. MDPI 2021-08-26 /pmc/articles/PMC8468791/ /pubmed/34574112 http://dx.doi.org/10.3390/foods10092002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zoz, Fiona
Guyot, Stéphane
Grandvalet, Cosette
Ragon, Mélanie
Lesniewska, Eric
Dupont, Sébastien
Firmesse, Olivier
Carpentier, Brigitte
Beney, Laurent
Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title_full Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title_fullStr Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title_full_unstemmed Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title_short Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope
title_sort management of listeria monocytogenes on surfaces via relative air humidity: key role of cell envelope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468791/
https://www.ncbi.nlm.nih.gov/pubmed/34574112
http://dx.doi.org/10.3390/foods10092002
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