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FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor

The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypH...

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Detalles Bibliográficos
Autores principales: Goryashchenko, Alexander S., Pakhomov, Alexey A., Ryabova, Anastasia V., Romanishkin, Igor D., Maksimov, Eugene G., Orsa, Alexander N., Serova, Oxana V., Mozhaev, Andrey A., Maksimova, Margarita A., Martynov, Vladimir I., Petrenko, Alexander G., Deyev, Igor E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468847/
https://www.ncbi.nlm.nih.gov/pubmed/34562930
http://dx.doi.org/10.3390/bios11090340

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