Characterization and Evaluation of Layered Bi(2)WO(6) Nanosheets as a New Antibacterial Agent

Background: Pathogenic microorganisms are causing increasing cases of mortality and morbidity, along with alarming rates of ineffectiveness as a result of acquired antimicrobial resistance. Bi(2)WO(6) showed good potential to be used as an antibacterial substance when exposed to visible light. This study demonstrates for the first time the dimension-dependent antibacterial activity of layered Bi(2)WO(6) nanosheets. Materials and methods: The synthesized layered Bi(2)WO(6) nanosheets were prepared by the hydrothermal method and characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and Raman and Fourier transform infrared spectroscopy (FTIR). Antibacterial and antibiotic-modulation activities were performed in triplicate by the microdilution method associated with visible light irradiation (LEDs). Results: Bi(2)WO(6) nanosheets were effective against all types of bacteria tested, with MIC values of 256 μg/mL against Escherichia coli standard and resistant strains, and 256 μg/mL and 32 μg/mL against Staphylococcus aureus standard and resistant strains, respectively. Two-dimensional (2D) Bi(2)WO(6) nanosheets showed antibacterial efficiency against both strains studied without the presence of light. Conclusions: Layered Bi(2)WO(6) nanosheets revealed dimension-dependent antibacterial activity of the Bi(2)WO(6) system.