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Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus

“Hongtuozhusun” (Phallus rubrovolvatus) is an important edible and medicinal mushroom endemic to Southwest China. However, yellow rot disease is a severe disease of P. rubrovolvatus that occurs extensively in Guizhou Province. It has caused major economic losses and hinders the development of the P....

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Autores principales: Yuan, Xiaoxiao, Peng, Keqin, Li, Changtian, Zhao, Zhibo, Zeng, Xiangyu, Tian, Fenghua, Li, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468998/
https://www.ncbi.nlm.nih.gov/pubmed/34575745
http://dx.doi.org/10.3390/jof7090707
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author Yuan, Xiaoxiao
Peng, Keqin
Li, Changtian
Zhao, Zhibo
Zeng, Xiangyu
Tian, Fenghua
Li, Yu
author_facet Yuan, Xiaoxiao
Peng, Keqin
Li, Changtian
Zhao, Zhibo
Zeng, Xiangyu
Tian, Fenghua
Li, Yu
author_sort Yuan, Xiaoxiao
collection PubMed
description “Hongtuozhusun” (Phallus rubrovolvatus) is an important edible and medicinal mushroom endemic to Southwest China. However, yellow rot disease is a severe disease of P. rubrovolvatus that occurs extensively in Guizhou Province. It has caused major economic losses and hinders the development of the P. rubrovolvatus industry. In this study, 28 microorganism strains were isolated from diseased fruiting bodies of P. rubrovolvatus at various stages, two of which were confirmed to be pathogenic based on Koch’s postulates. These two strains are introduced herein as Saccharomycopsis phalluae sp. nov. based on morphological, physiological, and molecular analysis. We reported a high-quality de novo sequencing and assembly of the S. phalluae genome using single-molecule real-time sequencing technology. The whole genome was approximately 14.148 Mb with a G+C content of 43.55%. Genome assembly generated 8 contigs with an N50 length of 1,822,654 bp. The genome comprised 5966 annotated protein-coding genes. This is the first report of mushroom disease caused by Saccharomycopsis species. We expect that the information on genome properties, particularly in pathogenicity-related genes, assist in developing effective control measures in order to prevent severe losses and make amendments in management strategies.
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spelling pubmed-84689982021-09-27 Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus Yuan, Xiaoxiao Peng, Keqin Li, Changtian Zhao, Zhibo Zeng, Xiangyu Tian, Fenghua Li, Yu J Fungi (Basel) Article “Hongtuozhusun” (Phallus rubrovolvatus) is an important edible and medicinal mushroom endemic to Southwest China. However, yellow rot disease is a severe disease of P. rubrovolvatus that occurs extensively in Guizhou Province. It has caused major economic losses and hinders the development of the P. rubrovolvatus industry. In this study, 28 microorganism strains were isolated from diseased fruiting bodies of P. rubrovolvatus at various stages, two of which were confirmed to be pathogenic based on Koch’s postulates. These two strains are introduced herein as Saccharomycopsis phalluae sp. nov. based on morphological, physiological, and molecular analysis. We reported a high-quality de novo sequencing and assembly of the S. phalluae genome using single-molecule real-time sequencing technology. The whole genome was approximately 14.148 Mb with a G+C content of 43.55%. Genome assembly generated 8 contigs with an N50 length of 1,822,654 bp. The genome comprised 5966 annotated protein-coding genes. This is the first report of mushroom disease caused by Saccharomycopsis species. We expect that the information on genome properties, particularly in pathogenicity-related genes, assist in developing effective control measures in order to prevent severe losses and make amendments in management strategies. MDPI 2021-08-28 /pmc/articles/PMC8468998/ /pubmed/34575745 http://dx.doi.org/10.3390/jof7090707 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Xiaoxiao
Peng, Keqin
Li, Changtian
Zhao, Zhibo
Zeng, Xiangyu
Tian, Fenghua
Li, Yu
Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title_full Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title_fullStr Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title_full_unstemmed Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title_short Complete Genomic Characterization and Identification of Saccharomycopsis phalluae sp. nov., a Novel Pathogen Causes Yellow Rot Disease on Phallus rubrovolvatus
title_sort complete genomic characterization and identification of saccharomycopsis phalluae sp. nov., a novel pathogen causes yellow rot disease on phallus rubrovolvatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468998/
https://www.ncbi.nlm.nih.gov/pubmed/34575745
http://dx.doi.org/10.3390/jof7090707
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