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IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function

Although it had been reported that Israeli acute paralysis virus (IAPV) can cause systemic infection in honey bees, little is known about how it establishes this infection and results in the typical symptoms, paralysis and trembling. Here, we used our previously constructed IAPV infectious clone to...

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Autores principales: Deng, Yanchun, Yang, Sa, Zhao, Hongxia, Diao, Qingyun, Hou, Chunsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469059/
https://www.ncbi.nlm.nih.gov/pubmed/34576241
http://dx.doi.org/10.3390/ijms221810078
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author Deng, Yanchun
Yang, Sa
Zhao, Hongxia
Diao, Qingyun
Hou, Chunsheng
author_facet Deng, Yanchun
Yang, Sa
Zhao, Hongxia
Diao, Qingyun
Hou, Chunsheng
author_sort Deng, Yanchun
collection PubMed
description Although it had been reported that Israeli acute paralysis virus (IAPV) can cause systemic infection in honey bees, little is known about how it establishes this infection and results in the typical symptoms, paralysis and trembling. Here, we used our previously constructed IAPV infectious clone to investigate viral loads in different tissues of honey bees and further identify the relation between tissue tropism and paralytic symptoms. Our results showed that tracheae showed a greater concentration of viral abundance than other tissues. The abundance of viral protein in the tracheae was positively associated with viral titers, and was further confirmed by immunological and ultrastructural evidence. Furthermore, higher viral loads in tracheae induced remarkable down-regulation of succinate dehydrogenase and cytochrome c oxidase genes, and progressed to causing respiratory failure of honey bees, resulting in the appearance of typical symptoms, paralysis and body trembling. Our results showed that paralysis symptoms or trembling was actually to mitigate tachypnea induced by IAPV infection due to the impairment of honey bee tracheae, and revealed a direct causal link between paralysis symptoms and tissue tropism. These findings provide new insights into the understanding of the underlying mechanism of paralysis symptoms of honey bees after viral infection and have implications for viral disease prevention and specific therapeutics in practice.
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spelling pubmed-84690592021-09-27 IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function Deng, Yanchun Yang, Sa Zhao, Hongxia Diao, Qingyun Hou, Chunsheng Int J Mol Sci Communication Although it had been reported that Israeli acute paralysis virus (IAPV) can cause systemic infection in honey bees, little is known about how it establishes this infection and results in the typical symptoms, paralysis and trembling. Here, we used our previously constructed IAPV infectious clone to investigate viral loads in different tissues of honey bees and further identify the relation between tissue tropism and paralytic symptoms. Our results showed that tracheae showed a greater concentration of viral abundance than other tissues. The abundance of viral protein in the tracheae was positively associated with viral titers, and was further confirmed by immunological and ultrastructural evidence. Furthermore, higher viral loads in tracheae induced remarkable down-regulation of succinate dehydrogenase and cytochrome c oxidase genes, and progressed to causing respiratory failure of honey bees, resulting in the appearance of typical symptoms, paralysis and body trembling. Our results showed that paralysis symptoms or trembling was actually to mitigate tachypnea induced by IAPV infection due to the impairment of honey bee tracheae, and revealed a direct causal link between paralysis symptoms and tissue tropism. These findings provide new insights into the understanding of the underlying mechanism of paralysis symptoms of honey bees after viral infection and have implications for viral disease prevention and specific therapeutics in practice. MDPI 2021-09-17 /pmc/articles/PMC8469059/ /pubmed/34576241 http://dx.doi.org/10.3390/ijms221810078 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Deng, Yanchun
Yang, Sa
Zhao, Hongxia
Diao, Qingyun
Hou, Chunsheng
IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title_full IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title_fullStr IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title_full_unstemmed IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title_short IAPV-Induced Paralytic Symptoms Associated with Tachypnea via Impaired Tracheal System Function
title_sort iapv-induced paralytic symptoms associated with tachypnea via impaired tracheal system function
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469059/
https://www.ncbi.nlm.nih.gov/pubmed/34576241
http://dx.doi.org/10.3390/ijms221810078
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