Cargando…
MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unr...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469245/ https://www.ncbi.nlm.nih.gov/pubmed/34575699 http://dx.doi.org/10.3390/jpm11090922 |
_version_ | 1784573881360580608 |
---|---|
author | Frye, Richard E. Rose, Shannon McCullough, Sandra Bennuri, Sirish C. Porter-Gill, Patricia A. Dweep, Harsh Gill, Pritmohinder S. |
author_facet | Frye, Richard E. Rose, Shannon McCullough, Sandra Bennuri, Sirish C. Porter-Gill, Patricia A. Dweep, Harsh Gill, Pritmohinder S. |
author_sort | Frye, Richard E. |
collection | PubMed |
description | Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unrelated age- and gender-matched controls and 10 typically developing siblings. Small RNAseq analysis identified miRNAs, and selected miRNAs were validated using quantitative real-time polymerase reaction (qRT-PCR). KEGG analysis identified target pathways, and selected predicted mRNAs were validated using qRT-PCR. Results: Small RNAseq analysis identified that multiple miRNAs differentiated ASD from unrelated controls and ASD from typically developing siblings, with only one, hsa-miR-451a_R-1, being in common. Verification with qRT-PCR showed that miR-320a differentiated ASD from both sibling and unrelated controls and that several members of the miR-181 family differentiated ASD from unrelated controls. Differential expression of AKT2, AKT3, TNF α and CamKinase II predicted by KEGG analysis was verified by qRT-PCR. Expression of CamKinase II βwas found to be correlated with the severity of stereotyped behavior of the ASD participants. Conclusions: This study provides insight into the mechanisms regulating molecular pathways in individuals with ASD and identifies differentiated regulated genes involved in both the central nervous system and the immune system. |
format | Online Article Text |
id | pubmed-8469245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84692452021-09-27 MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation Frye, Richard E. Rose, Shannon McCullough, Sandra Bennuri, Sirish C. Porter-Gill, Patricia A. Dweep, Harsh Gill, Pritmohinder S. J Pers Med Article Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unrelated age- and gender-matched controls and 10 typically developing siblings. Small RNAseq analysis identified miRNAs, and selected miRNAs were validated using quantitative real-time polymerase reaction (qRT-PCR). KEGG analysis identified target pathways, and selected predicted mRNAs were validated using qRT-PCR. Results: Small RNAseq analysis identified that multiple miRNAs differentiated ASD from unrelated controls and ASD from typically developing siblings, with only one, hsa-miR-451a_R-1, being in common. Verification with qRT-PCR showed that miR-320a differentiated ASD from both sibling and unrelated controls and that several members of the miR-181 family differentiated ASD from unrelated controls. Differential expression of AKT2, AKT3, TNF α and CamKinase II predicted by KEGG analysis was verified by qRT-PCR. Expression of CamKinase II βwas found to be correlated with the severity of stereotyped behavior of the ASD participants. Conclusions: This study provides insight into the mechanisms regulating molecular pathways in individuals with ASD and identifies differentiated regulated genes involved in both the central nervous system and the immune system. MDPI 2021-09-17 /pmc/articles/PMC8469245/ /pubmed/34575699 http://dx.doi.org/10.3390/jpm11090922 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Frye, Richard E. Rose, Shannon McCullough, Sandra Bennuri, Sirish C. Porter-Gill, Patricia A. Dweep, Harsh Gill, Pritmohinder S. MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title | MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title_full | MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title_fullStr | MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title_full_unstemmed | MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title_short | MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation |
title_sort | microrna expression profiles in autism spectrum disorder: role for mir-181 in immunomodulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469245/ https://www.ncbi.nlm.nih.gov/pubmed/34575699 http://dx.doi.org/10.3390/jpm11090922 |
work_keys_str_mv | AT fryericharde micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT roseshannon micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT mcculloughsandra micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT bennurisirishc micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT portergillpatriciaa micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT dweepharsh micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation AT gillpritmohinders micrornaexpressionprofilesinautismspectrumdisorderroleformir181inimmunomodulation |