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MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation

Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unr...

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Autores principales: Frye, Richard E., Rose, Shannon, McCullough, Sandra, Bennuri, Sirish C., Porter-Gill, Patricia A., Dweep, Harsh, Gill, Pritmohinder S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469245/
https://www.ncbi.nlm.nih.gov/pubmed/34575699
http://dx.doi.org/10.3390/jpm11090922
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author Frye, Richard E.
Rose, Shannon
McCullough, Sandra
Bennuri, Sirish C.
Porter-Gill, Patricia A.
Dweep, Harsh
Gill, Pritmohinder S.
author_facet Frye, Richard E.
Rose, Shannon
McCullough, Sandra
Bennuri, Sirish C.
Porter-Gill, Patricia A.
Dweep, Harsh
Gill, Pritmohinder S.
author_sort Frye, Richard E.
collection PubMed
description Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unrelated age- and gender-matched controls and 10 typically developing siblings. Small RNAseq analysis identified miRNAs, and selected miRNAs were validated using quantitative real-time polymerase reaction (qRT-PCR). KEGG analysis identified target pathways, and selected predicted mRNAs were validated using qRT-PCR. Results: Small RNAseq analysis identified that multiple miRNAs differentiated ASD from unrelated controls and ASD from typically developing siblings, with only one, hsa-miR-451a_R-1, being in common. Verification with qRT-PCR showed that miR-320a differentiated ASD from both sibling and unrelated controls and that several members of the miR-181 family differentiated ASD from unrelated controls. Differential expression of AKT2, AKT3, TNF α and CamKinase II predicted by KEGG analysis was verified by qRT-PCR. Expression of CamKinase II βwas found to be correlated with the severity of stereotyped behavior of the ASD participants. Conclusions: This study provides insight into the mechanisms regulating molecular pathways in individuals with ASD and identifies differentiated regulated genes involved in both the central nervous system and the immune system.
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spelling pubmed-84692452021-09-27 MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation Frye, Richard E. Rose, Shannon McCullough, Sandra Bennuri, Sirish C. Porter-Gill, Patricia A. Dweep, Harsh Gill, Pritmohinder S. J Pers Med Article Background: MicroRNAs (miRNAs) are important regulators of molecular pathways in psychiatric disease. Here, we examine differential miRNAs expression in lymphoblastoid cell lines (LCLs) derived from 10 individuals with autism spectrum disorder (ASD) and compare them to seven typically developing unrelated age- and gender-matched controls and 10 typically developing siblings. Small RNAseq analysis identified miRNAs, and selected miRNAs were validated using quantitative real-time polymerase reaction (qRT-PCR). KEGG analysis identified target pathways, and selected predicted mRNAs were validated using qRT-PCR. Results: Small RNAseq analysis identified that multiple miRNAs differentiated ASD from unrelated controls and ASD from typically developing siblings, with only one, hsa-miR-451a_R-1, being in common. Verification with qRT-PCR showed that miR-320a differentiated ASD from both sibling and unrelated controls and that several members of the miR-181 family differentiated ASD from unrelated controls. Differential expression of AKT2, AKT3, TNF α and CamKinase II predicted by KEGG analysis was verified by qRT-PCR. Expression of CamKinase II βwas found to be correlated with the severity of stereotyped behavior of the ASD participants. Conclusions: This study provides insight into the mechanisms regulating molecular pathways in individuals with ASD and identifies differentiated regulated genes involved in both the central nervous system and the immune system. MDPI 2021-09-17 /pmc/articles/PMC8469245/ /pubmed/34575699 http://dx.doi.org/10.3390/jpm11090922 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Frye, Richard E.
Rose, Shannon
McCullough, Sandra
Bennuri, Sirish C.
Porter-Gill, Patricia A.
Dweep, Harsh
Gill, Pritmohinder S.
MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title_full MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title_fullStr MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title_full_unstemmed MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title_short MicroRNA Expression Profiles in Autism Spectrum Disorder: Role for miR-181 in Immunomodulation
title_sort microrna expression profiles in autism spectrum disorder: role for mir-181 in immunomodulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469245/
https://www.ncbi.nlm.nih.gov/pubmed/34575699
http://dx.doi.org/10.3390/jpm11090922
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