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MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes
The compact nucleosomal structure limits DNA accessibility and regulates DNA-dependent cellular activities. Linker histones bind to nucleosomes and compact nucleosomal arrays into a higher-order chromatin structure. Recent developments in high throughput technologies and structural computational stu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469290/ https://www.ncbi.nlm.nih.gov/pubmed/34571888 http://dx.doi.org/10.3390/cells10092239 |
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author | Shen, Chang-Hui Allan, James |
author_facet | Shen, Chang-Hui Allan, James |
author_sort | Shen, Chang-Hui |
collection | PubMed |
description | The compact nucleosomal structure limits DNA accessibility and regulates DNA-dependent cellular activities. Linker histones bind to nucleosomes and compact nucleosomal arrays into a higher-order chromatin structure. Recent developments in high throughput technologies and structural computational studies provide nucleosome positioning at a high resolution and contribute to the information of linker histone location within a chromatosome. However, the precise linker histone location within the chromatin fibre remains unclear. Using monomer extension, we mapped core particle and chromatosomal positions over a core histone-reconstituted, 1.5 kb stretch of DNA from the chicken adult β-globin gene, after titration with linker histones and linker histone globular domains. Our results show that, although linker histone globular domains and linker histones display a wide variation in their binding affinity for different positioned nucleosomes, they do not alter nucleosome positions or generate new nucleosome positions. Furthermore, the extra ~20 bp of DNA protected in a chromatosome is usually symmetrically distributed at each end of the core particle, suggesting linker histones or linker histone globular domains are located close to the nucleosomal dyad axis. |
format | Online Article Text |
id | pubmed-8469290 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84692902021-09-27 MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes Shen, Chang-Hui Allan, James Cells Article The compact nucleosomal structure limits DNA accessibility and regulates DNA-dependent cellular activities. Linker histones bind to nucleosomes and compact nucleosomal arrays into a higher-order chromatin structure. Recent developments in high throughput technologies and structural computational studies provide nucleosome positioning at a high resolution and contribute to the information of linker histone location within a chromatosome. However, the precise linker histone location within the chromatin fibre remains unclear. Using monomer extension, we mapped core particle and chromatosomal positions over a core histone-reconstituted, 1.5 kb stretch of DNA from the chicken adult β-globin gene, after titration with linker histones and linker histone globular domains. Our results show that, although linker histone globular domains and linker histones display a wide variation in their binding affinity for different positioned nucleosomes, they do not alter nucleosome positions or generate new nucleosome positions. Furthermore, the extra ~20 bp of DNA protected in a chromatosome is usually symmetrically distributed at each end of the core particle, suggesting linker histones or linker histone globular domains are located close to the nucleosomal dyad axis. MDPI 2021-08-29 /pmc/articles/PMC8469290/ /pubmed/34571888 http://dx.doi.org/10.3390/cells10092239 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shen, Chang-Hui Allan, James MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title | MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title_full | MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title_fullStr | MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title_full_unstemmed | MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title_short | MNase Digestion Protection Patterns of the Linker DNA in Chromatosomes |
title_sort | mnase digestion protection patterns of the linker dna in chromatosomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469290/ https://www.ncbi.nlm.nih.gov/pubmed/34571888 http://dx.doi.org/10.3390/cells10092239 |
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