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Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.)
Members of the IQM (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the IQM gene family and their expression profiles under diverse biotic and abiotic stresses, we identified 8 IQM genes in the r...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469326/ https://www.ncbi.nlm.nih.gov/pubmed/34579481 http://dx.doi.org/10.3390/plants10091949 |
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author | Fan, Tian Lv, Tianxiao Xie, Chuping Zhou, Yuping Tian, Changen |
author_facet | Fan, Tian Lv, Tianxiao Xie, Chuping Zhou, Yuping Tian, Changen |
author_sort | Fan, Tian |
collection | PubMed |
description | Members of the IQM (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the IQM gene family and their expression profiles under diverse biotic and abiotic stresses, we identified 8 IQM genes in the rice genome. In the current study, the whole genome identification and characterization of OsIQMs, including the gene and protein structure, genome localization, phylogenetic relationship, gene expression and yeast two-hybrid were performed. Eight IQM genes were classified into three subfamilies (I–III) according to the phylogenetic analysis. Gene structure and protein motif analyses showed that these IQM genes are relatively conserved within each subfamily of rice. The 8 OsIQM genes are distributed on seven out of the twelve chromosomes, with three IQM gene pairs involved in segmental duplication events. The evolutionary patterns analysis revealed that the IQM genes underwent a large-scale event within the last 20 to 9 million years. In addition, quantitative real-time PCR analysis of eight OsIQMs genes displayed different expression patterns at different developmental stages and in different tissues as well as showed that most IQM genes were responsive to PEG, NaCl, jasmonic acid (JA), abscisic acid (ABA) treatment, suggesting their crucial roles in biotic, and abiotic stress response. Additionally, a yeast two-hybrid assay showed that OsIQMs can interact with OsCaMs, and the IQ motif of OsIQMs is required for OsIQMs to combine with OsCaMs. Our results will be valuable to further characterize the important biological functions of rice IQM genes. |
format | Online Article Text |
id | pubmed-8469326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84693262021-09-27 Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) Fan, Tian Lv, Tianxiao Xie, Chuping Zhou, Yuping Tian, Changen Plants (Basel) Article Members of the IQM (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the IQM gene family and their expression profiles under diverse biotic and abiotic stresses, we identified 8 IQM genes in the rice genome. In the current study, the whole genome identification and characterization of OsIQMs, including the gene and protein structure, genome localization, phylogenetic relationship, gene expression and yeast two-hybrid were performed. Eight IQM genes were classified into three subfamilies (I–III) according to the phylogenetic analysis. Gene structure and protein motif analyses showed that these IQM genes are relatively conserved within each subfamily of rice. The 8 OsIQM genes are distributed on seven out of the twelve chromosomes, with three IQM gene pairs involved in segmental duplication events. The evolutionary patterns analysis revealed that the IQM genes underwent a large-scale event within the last 20 to 9 million years. In addition, quantitative real-time PCR analysis of eight OsIQMs genes displayed different expression patterns at different developmental stages and in different tissues as well as showed that most IQM genes were responsive to PEG, NaCl, jasmonic acid (JA), abscisic acid (ABA) treatment, suggesting their crucial roles in biotic, and abiotic stress response. Additionally, a yeast two-hybrid assay showed that OsIQMs can interact with OsCaMs, and the IQ motif of OsIQMs is required for OsIQMs to combine with OsCaMs. Our results will be valuable to further characterize the important biological functions of rice IQM genes. MDPI 2021-09-18 /pmc/articles/PMC8469326/ /pubmed/34579481 http://dx.doi.org/10.3390/plants10091949 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fan, Tian Lv, Tianxiao Xie, Chuping Zhou, Yuping Tian, Changen Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title | Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title_full | Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title_fullStr | Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title_full_unstemmed | Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title_short | Genome-Wide Analysis of the IQM Gene Family in Rice (Oryza sativa L.) |
title_sort | genome-wide analysis of the iqm gene family in rice (oryza sativa l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469326/ https://www.ncbi.nlm.nih.gov/pubmed/34579481 http://dx.doi.org/10.3390/plants10091949 |
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