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Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval

Escherichia coli causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of E. coli isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptib...

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Autores principales: Lin, Wei-Hung, Zhang, Yen-Zhen, Liu, Po-Yao, Chen, Po-Shun, Wang, Shining, Kuo, Pei-Yun, Thuy, Tran Thi Dieu, Duong, Tran Thi Thuy, Wen, Li-Li, Hsieh, Yi-Hsien, Wang, Ming-Cheng, Kao, Cheng-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469484/
https://www.ncbi.nlm.nih.gov/pubmed/34578189
http://dx.doi.org/10.3390/pathogens10091156
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author Lin, Wei-Hung
Zhang, Yen-Zhen
Liu, Po-Yao
Chen, Po-Shun
Wang, Shining
Kuo, Pei-Yun
Thuy, Tran Thi Dieu
Duong, Tran Thi Thuy
Wen, Li-Li
Hsieh, Yi-Hsien
Wang, Ming-Cheng
Kao, Cheng-Yen
author_facet Lin, Wei-Hung
Zhang, Yen-Zhen
Liu, Po-Yao
Chen, Po-Shun
Wang, Shining
Kuo, Pei-Yun
Thuy, Tran Thi Dieu
Duong, Tran Thi Thuy
Wen, Li-Li
Hsieh, Yi-Hsien
Wang, Ming-Cheng
Kao, Cheng-Yen
author_sort Lin, Wei-Hung
collection PubMed
description Escherichia coli causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of E. coli isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of E. coli was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. E. coli isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-E. coli in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-E. coli were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes aer and fimH were higher in E. coli in 2009–2010 compared to those in 2020. In contrast, afa and sat showed higher frequencies in E. coli isolates in 2020 compared to E. coli in 2009–2010.
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spelling pubmed-84694842021-09-27 Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval Lin, Wei-Hung Zhang, Yen-Zhen Liu, Po-Yao Chen, Po-Shun Wang, Shining Kuo, Pei-Yun Thuy, Tran Thi Dieu Duong, Tran Thi Thuy Wen, Li-Li Hsieh, Yi-Hsien Wang, Ming-Cheng Kao, Cheng-Yen Pathogens Article Escherichia coli causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of E. coli isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of E. coli was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. E. coli isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-E. coli in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-E. coli were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes aer and fimH were higher in E. coli in 2009–2010 compared to those in 2020. In contrast, afa and sat showed higher frequencies in E. coli isolates in 2020 compared to E. coli in 2009–2010. MDPI 2021-09-08 /pmc/articles/PMC8469484/ /pubmed/34578189 http://dx.doi.org/10.3390/pathogens10091156 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Wei-Hung
Zhang, Yen-Zhen
Liu, Po-Yao
Chen, Po-Shun
Wang, Shining
Kuo, Pei-Yun
Thuy, Tran Thi Dieu
Duong, Tran Thi Thuy
Wen, Li-Li
Hsieh, Yi-Hsien
Wang, Ming-Cheng
Kao, Cheng-Yen
Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title_full Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title_fullStr Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title_full_unstemmed Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title_short Distinct Characteristics of Escherichia coli Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
title_sort distinct characteristics of escherichia coli isolated from patients with urinary tract infections in a medical center at a ten-year interval
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469484/
https://www.ncbi.nlm.nih.gov/pubmed/34578189
http://dx.doi.org/10.3390/pathogens10091156
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