Cargando…

Enhancement of Transgene Expression by Mild Hypothermia Is Promoter Dependent in HEK293 Cells

Mild hypothermia has been widely used to enhance transgene expression and improve the cellular productivity of mammalian cells. This study investigated mild hypothermia-responsive exogenous promoters in human embryonic kidney 293 (HEK293) cells using site-specific integration of various promoter seq...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jang, Min Ho, Min, Honggi, Lee, Jae Seong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469586/ https://www.ncbi.nlm.nih.gov/pubmed/34575051 http://dx.doi.org/10.3390/life11090901

Ejemplares similares

The CAG promoter maintains high‐level transgene expression in HEK293 cells
por: Dou, Yuanyuan, et al.
Publicado: (2020)

Lack of current observed in HEK293 cells expressing NALCN channels
por: Egan, Jennifer M., et al.
Publicado: (2018)

Effect of promoter, promoter mutation and enhancer on transgene expression mediated by episomal vectors in transfected HEK293, Chang liver and primary cells
por: Xu, Zhong-Jie, et al.
Publicado: (2019)

Microtubule Integrity Is Associated with the Functional Activity of Mitochondria in HEK293
por: Cho, Min Jeong, et al.
Publicado: (2021)

Lentiviral Vectors Mediate Long-Term and High Efficiency Transgene Expression in HEK 293T cells
por: Mao, Yingying, et al.
Publicado: (2015)

Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
por: Lin, Chi-Yen, et al.
Publicado: (2015)

Slight Variations in the Sequence Downstream of the Polyadenylation Signal Significantly Increase Transgene Expression in HEK293T and CHO Cells
por: Omelina, Evgeniya S., et al.
Publicado: (2022)

Effect of PTTG on endogenous gene expression in HEK 293 cells
por: Panguluri, Siva K, et al.
Publicado: (2009)

Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine
por: Mirzaie, Vida, et al.
Publicado: (2020)

HEK293T Cells Are Heterozygous for CCR5 Delta 32 Mutation
por: Qi, Chunxia, et al.
Publicado: (2016)

Using HEK293T Expression System to Study Photoactive Plant Cryptochromes
por: Yang, Liang, et al.
Publicado: (2016)

Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
por: MITOMA, Shuya, et al.
Publicado: (2020)

Cholesterol Down-Regulates BK Channels Stably Expressed in HEK 293 Cells
por: Wu, Wei, et al.
Publicado: (2013)

Cloning, Expression and Characterization of Zebra Fish Ferroportin in Hek 293T Cell Line
por: Rafiee, A, et al.
Publicado: (2012)

Allitridi Inhibits Multiple Cardiac Potassium Channels Expressed in HEK 293 Cells
por: Xu, Xiao-Hui, et al.
Publicado: (2012)

Effects of equol on multiple K(+) channels stably expressed in HEK 293 cells
por: Deng, Xiu-Ling, et al.
Publicado: (2017)

Patulin suppresses α(1)-adrenergic receptor expression in HEK293 cells
por: Pillay, Yashodani, et al.
Publicado: (2020)

Inducible HEK293 AAV packaging cell lines expressing Rep proteins
por: Jalšić, Lovro, et al.
Publicado: (2023)

Generation of Recombinant Antibodies in HEK293F Cells for the Detection of Staphylococcus aureus
por: Kim, Joo-Kyung, et al.
Publicado: (2022)

HEK293S Cells Have Functional Retinoid Processing Machinery
por: Brueggemann, Lioubov I., et al.
Publicado: (2002)

Effects of endotoxin on pacemaker funny current in HEK 293 cells
por: Papaioannou, VP, et al.
Publicado: (2011)

Echinacea-induced cytosolic Ca(2+ )elevation in HEK293
por: Wu, Lankun, et al.
Publicado: (2010)

Analysis of DNA topology of EBV minichromosomes in HEK 293 cells
por: Castán, Alicia, et al.
Publicado: (2017)

HEK293T cell lines defective for O-linked glycosylation
por: Termini, James M., et al.
Publicado: (2017)

Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay
por: Reischmann, Patricia, et al.
Publicado: (2015)

Time-Dependent Effects of Anesthetic Isoflurane on Reactive Oxygen Species Levels in HEK-293 Cells
por: Sun, Yongxing, et al.
Publicado: (2014)

Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems
por: Thompson, Christine M, et al.
Publicado: (2015)

DAPIT Over-Expression Modulates Glucose Metabolism and Cell Behaviour in HEK293T Cells
por: Kontro, Heidi, et al.
Publicado: (2015)

A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization
por: Ooi, Amanda, et al.
Publicado: (2016)

Inhibitory effects and mechanism of dihydroberberine on hERG channels expressed in HEK293 cells
por: Yu, Dahai, et al.
Publicado: (2017)

Analysis of Circular RNA Expression Profile in HEK 293T Cells Exposed to Ionizing Radiation
por: He, Ningning, et al.
Publicado: (2019)

A human expression system based on HEK293 for the stable production of recombinant erythropoietin
por: Chin, Christine Lin, et al.
Publicado: (2019)

Development of an experimental method of systematically estimating protein expression limits in HEK293 cells
por: Mori, Yoshihiro, et al.
Publicado: (2020)

Generation and utilization of a HEK-293T murine GM-CSF expressing cell line
por: Robinson, Elektra Kantzari, et al.
Publicado: (2021)

Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
por: Abaandou, Laura, et al.
Publicado: (2021)

Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293
por: Porosk, Ly, et al.
Publicado: (2022)

Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in Diagnostics
por: Powers, Jordan A., et al.
Publicado: (2022)

Immunogenicity and Antigenicity of the Ectodomain of Rabies Virus Glycoprotein Stably Expressed in HEK293T Cells
por: Li, Qingqing, et al.
Publicado: (2023)

Detection of Recombinant Proteins SOX2 and OCT4 Interacting in HEK293T Cells Using Real-Time Quantitative PCR
por: Kanayev, Darkhan, et al.
Publicado: (2022)

Cytosolic domain regulates the calcium sensitivity and surface expression of BEST1 channels in the HEK293 cells
por: Kim, Kwon Woo, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_mf2ehdi1ue18uc2lmudhgk3qqk