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Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice
Homer represents a diversified family of scaffold and transduction proteins made up of several isoforms. Here, we present preliminary observations on skeletal muscle adaptation and plasticity in a transgenic model of Homer 2(-/-) mouse using a multifaceted approach entailing morphometry, quantitativ...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469648/ https://www.ncbi.nlm.nih.gov/pubmed/34564458 http://dx.doi.org/10.3390/metabo11090642 |
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author | Lorenzon, Paola Furlan, Sandra Ravara, Barbara Bosutti, Alessandra Massaria, Gabriele Bernareggi, Annalisa Sciancalepore, Marina Trautmann, Gabor Block, Katharina Blottner, Dieter Worley, Paul F. Zampieri, Sandra Salanova, Michele Volpe, Pompeo |
author_facet | Lorenzon, Paola Furlan, Sandra Ravara, Barbara Bosutti, Alessandra Massaria, Gabriele Bernareggi, Annalisa Sciancalepore, Marina Trautmann, Gabor Block, Katharina Blottner, Dieter Worley, Paul F. Zampieri, Sandra Salanova, Michele Volpe, Pompeo |
author_sort | Lorenzon, Paola |
collection | PubMed |
description | Homer represents a diversified family of scaffold and transduction proteins made up of several isoforms. Here, we present preliminary observations on skeletal muscle adaptation and plasticity in a transgenic model of Homer 2(-/-) mouse using a multifaceted approach entailing morphometry, quantitative RT-PCR (Reverse Transcription PCR), confocal immunofluorescence, and electrophysiology. Morphometry shows that Soleus muscle (SOL), at variance with Extensor digitorum longus muscle (EDL) and Flexor digitorum brevis muscle (FDB), displays sizable reduction of fibre cross-sectional area compared to the WT counterparts. In SOL of Homer 2(-/-) mice, quantitative RT-PCR indicated the upregulation of Atrogin-1 and Muscle ring finger protein 1 (MuRF1) genes, and confocal immunofluorescence showed the decrease of neuromuscular junction (NMJ) Homer content. Electrophysiological measurements of isolated FDB fibres from Homer 2(-/-) mice detected the exclusive presence of the adult ε-nAChR isoform excluding denervation. As for NMJ morphology, data were not conclusive, and further work is needed to ascertain whether the null Homer 2 phenotype induces any endplate remodelling. Within the context of adaptation and plasticity, the present data show that Homer 2 is a co-regulator of the normotrophic status in a muscle specific fashion. |
format | Online Article Text |
id | pubmed-8469648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84696482021-09-27 Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice Lorenzon, Paola Furlan, Sandra Ravara, Barbara Bosutti, Alessandra Massaria, Gabriele Bernareggi, Annalisa Sciancalepore, Marina Trautmann, Gabor Block, Katharina Blottner, Dieter Worley, Paul F. Zampieri, Sandra Salanova, Michele Volpe, Pompeo Metabolites Article Homer represents a diversified family of scaffold and transduction proteins made up of several isoforms. Here, we present preliminary observations on skeletal muscle adaptation and plasticity in a transgenic model of Homer 2(-/-) mouse using a multifaceted approach entailing morphometry, quantitative RT-PCR (Reverse Transcription PCR), confocal immunofluorescence, and electrophysiology. Morphometry shows that Soleus muscle (SOL), at variance with Extensor digitorum longus muscle (EDL) and Flexor digitorum brevis muscle (FDB), displays sizable reduction of fibre cross-sectional area compared to the WT counterparts. In SOL of Homer 2(-/-) mice, quantitative RT-PCR indicated the upregulation of Atrogin-1 and Muscle ring finger protein 1 (MuRF1) genes, and confocal immunofluorescence showed the decrease of neuromuscular junction (NMJ) Homer content. Electrophysiological measurements of isolated FDB fibres from Homer 2(-/-) mice detected the exclusive presence of the adult ε-nAChR isoform excluding denervation. As for NMJ morphology, data were not conclusive, and further work is needed to ascertain whether the null Homer 2 phenotype induces any endplate remodelling. Within the context of adaptation and plasticity, the present data show that Homer 2 is a co-regulator of the normotrophic status in a muscle specific fashion. MDPI 2021-09-19 /pmc/articles/PMC8469648/ /pubmed/34564458 http://dx.doi.org/10.3390/metabo11090642 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lorenzon, Paola Furlan, Sandra Ravara, Barbara Bosutti, Alessandra Massaria, Gabriele Bernareggi, Annalisa Sciancalepore, Marina Trautmann, Gabor Block, Katharina Blottner, Dieter Worley, Paul F. Zampieri, Sandra Salanova, Michele Volpe, Pompeo Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title | Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title_full | Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title_fullStr | Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title_full_unstemmed | Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title_short | Preliminary Observations on Skeletal Muscle Adaptation and Plasticity in Homer 2(-/-) Mice |
title_sort | preliminary observations on skeletal muscle adaptation and plasticity in homer 2(-/-) mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469648/ https://www.ncbi.nlm.nih.gov/pubmed/34564458 http://dx.doi.org/10.3390/metabo11090642 |
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