In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe

Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophis...

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Autores principales: Doppler, Philipp, Gasser, Christoph, Kriechbaum, Ricarda, Ferizi, Ardita, Spadiut, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469707/
https://www.ncbi.nlm.nih.gov/pubmed/34562950
http://dx.doi.org/10.3390/bioengineering8090129
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author Doppler, Philipp
Gasser, Christoph
Kriechbaum, Ricarda
Ferizi, Ardita
Spadiut, Oliver
author_facet Doppler, Philipp
Gasser, Christoph
Kriechbaum, Ricarda
Ferizi, Ardita
Spadiut, Oliver
author_sort Doppler, Philipp
collection PubMed
description Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors.
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spelling pubmed-84697072021-09-27 In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe Doppler, Philipp Gasser, Christoph Kriechbaum, Ricarda Ferizi, Ardita Spadiut, Oliver Bioengineering (Basel) Communication Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors. MDPI 2021-09-21 /pmc/articles/PMC8469707/ /pubmed/34562950 http://dx.doi.org/10.3390/bioengineering8090129 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Doppler, Philipp
Gasser, Christoph
Kriechbaum, Ricarda
Ferizi, Ardita
Spadiut, Oliver
In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title_full In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title_fullStr In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title_full_unstemmed In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title_short In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
title_sort in situ quantification of polyhydroxybutyrate in photobioreactor cultivations of synechocystis sp. using an ultrasound-enhanced atr-ftir spectroscopy probe
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469707/
https://www.ncbi.nlm.nih.gov/pubmed/34562950
http://dx.doi.org/10.3390/bioengineering8090129
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