Genome-Wide Association Study Reveals a New Quantitative Trait Locus in Rice Related to Resistance to Brown Planthopper Nilaparvata lugens (Stål)

SIMPLE SUMMARY: The brown planthopper Nilaparvata lugens (Stål) (BPH) is one of the main rice pests in Asian areas. The development of rice varieties harboring resistance genes is the most economical and effective method of managing BPH. In this study, 123 rice germplasms were identified for resistance and durable resistance by using the rice planthopper resistance identification system. Forty-two of the 123 rice varieties were classified as resistant to brown planthopper, and among them, twelve rice varieties had a long, durable resistance period. One potential durable resistance to brown planthopper locus on chromosome 2 was found by a genome-wide association study (GWAS). There are 13 candidate genes at this locus, and several of them are related to disease and pest resistance. Our study found a potential durable resistance locus to BPH, which has guiding significance for subsequent resistance breeding. ABSTRACT: The brown planthopper (BPH) is one of the main pests endangering rice yields. The development of rice varieties harboring resistance genes is the most economical and effective method of managing BPH. To identify new BPH resistance-related genes, a total of 123 rice varieties were assessed for resistance and durable resistance. Three varieties were immune, and nine were highly resistant to BPH. After whole-genome resequencing of all 123 varieties, 1,897,845 single nucleotide polymorphisms (SNPs) were identified. Linkage disequilibrium (LD) decay analysis showed that the average LD of the SNPs at 20 kb was 0.30 (r(2)) and attenuated to half value (~0.30) at a distance of about 233 kb. A genome-wide association study (GWAS) of durable resistance to BPH was conducted using the Fast-MLM model. One quantitative trait locus, identified on chromosome 2, included 13 candidate genes. Two candidate genes contained a leucine-rich repeat and CC-NBS-LRR or NB-ARC domains, which might confer resistance to pests or diseases. Interestingly, LOC_Os02g27540 was highly expressed and was induced by BPH; GWAS identified potential rice genes coding for durable resistance to BPH. This study helps to elucidate the mechanism of durable resistance to BPH in rice and provides essential genetic information for breeding and functional verification of resistant varieties.