Cargando…

Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens

Eggshell quality is economically important for table eggs and functionally indispensable for hatching eggs. During the formation of eggshell in the uterus, organic matrixes in uterine fluid can control and modify the formation of calcified eggshell. At present, there are limited studies focusing on...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xiqiong, Zhu, Ping, Sun, Zhihua, Zhang, Junnan, Sun, Congjiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469744/
https://www.ncbi.nlm.nih.gov/pubmed/34564391
http://dx.doi.org/10.3390/metabo11090575
_version_ 1784574014611521536
author Wang, Xiqiong
Zhu, Ping
Sun, Zhihua
Zhang, Junnan
Sun, Congjiao
author_facet Wang, Xiqiong
Zhu, Ping
Sun, Zhihua
Zhang, Junnan
Sun, Congjiao
author_sort Wang, Xiqiong
collection PubMed
description Eggshell quality is economically important for table eggs and functionally indispensable for hatching eggs. During the formation of eggshell in the uterus, organic matrixes in uterine fluid can control and modify the formation of calcified eggshell. At present, there are limited studies focusing on the effect of uterine organic metabolites on eggshell quality. In this study, an LC-MS-based metabolomic technology was performed to identify the crucial uterine metabolites that differently presented in hens producing eggs with divergent eggshell quality (eggshell strength, thickness, and weight). More than 1000 metabolites were identified in uterine fluid, and six putative metabolites, including phosphatidylcholine, diacylglycerol, verapamil, risedronate, coproporphyrinogen III, and biliverdin, were screened to play crucial roles in eggshell calcification. Then, two trials for oral administration and in vitro calcite crystal growth were conducted to verify the effect of potential different metabolites on the eggshell quality. Verapamil has a temporary effect on decreasing eggshell strength and eggshell thickness. Coproporphyrinogen III could induce smaller calcite crystals to improve eggshell strength while biliverdin could modify crystal morphology by forming rougher faces and rounder edges to strengthen the eggshell. The present study gives us new insight to understand the role of uterine fluid matrixes in eggshell calcification.
format Online
Article
Text
id pubmed-8469744
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84697442021-09-27 Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens Wang, Xiqiong Zhu, Ping Sun, Zhihua Zhang, Junnan Sun, Congjiao Metabolites Article Eggshell quality is economically important for table eggs and functionally indispensable for hatching eggs. During the formation of eggshell in the uterus, organic matrixes in uterine fluid can control and modify the formation of calcified eggshell. At present, there are limited studies focusing on the effect of uterine organic metabolites on eggshell quality. In this study, an LC-MS-based metabolomic technology was performed to identify the crucial uterine metabolites that differently presented in hens producing eggs with divergent eggshell quality (eggshell strength, thickness, and weight). More than 1000 metabolites were identified in uterine fluid, and six putative metabolites, including phosphatidylcholine, diacylglycerol, verapamil, risedronate, coproporphyrinogen III, and biliverdin, were screened to play crucial roles in eggshell calcification. Then, two trials for oral administration and in vitro calcite crystal growth were conducted to verify the effect of potential different metabolites on the eggshell quality. Verapamil has a temporary effect on decreasing eggshell strength and eggshell thickness. Coproporphyrinogen III could induce smaller calcite crystals to improve eggshell strength while biliverdin could modify crystal morphology by forming rougher faces and rounder edges to strengthen the eggshell. The present study gives us new insight to understand the role of uterine fluid matrixes in eggshell calcification. MDPI 2021-08-26 /pmc/articles/PMC8469744/ /pubmed/34564391 http://dx.doi.org/10.3390/metabo11090575 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xiqiong
Zhu, Ping
Sun, Zhihua
Zhang, Junnan
Sun, Congjiao
Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title_full Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title_fullStr Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title_full_unstemmed Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title_short Uterine Metabolomic Analysis for the Regulation of Eggshell Calcification in Chickens
title_sort uterine metabolomic analysis for the regulation of eggshell calcification in chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469744/
https://www.ncbi.nlm.nih.gov/pubmed/34564391
http://dx.doi.org/10.3390/metabo11090575
work_keys_str_mv AT wangxiqiong uterinemetabolomicanalysisfortheregulationofeggshellcalcificationinchickens
AT zhuping uterinemetabolomicanalysisfortheregulationofeggshellcalcificationinchickens
AT sunzhihua uterinemetabolomicanalysisfortheregulationofeggshellcalcificationinchickens
AT zhangjunnan uterinemetabolomicanalysisfortheregulationofeggshellcalcificationinchickens
AT suncongjiao uterinemetabolomicanalysisfortheregulationofeggshellcalcificationinchickens