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Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function
Helicases are enzymes that convert the chemical energy stored in ATP into mechanical work, allowing them to move along and manipulate nucleic acids. The helicase superfamily 1 (Sf1) is one of the largest subgroups of helicases and they are required for a range of cellular activities across all domai...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469786/ https://www.ncbi.nlm.nih.gov/pubmed/34573298 http://dx.doi.org/10.3390/genes12091319 |
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author | Meir, Aviv Greene, Eric C. |
author_facet | Meir, Aviv Greene, Eric C. |
author_sort | Meir, Aviv |
collection | PubMed |
description | Helicases are enzymes that convert the chemical energy stored in ATP into mechanical work, allowing them to move along and manipulate nucleic acids. The helicase superfamily 1 (Sf1) is one of the largest subgroups of helicases and they are required for a range of cellular activities across all domains of life. Sf1 helicases can be further subdivided into two classes called the Sf1a and Sf1b helicases, which move in opposite directions on nucleic acids. The results of this movement can range from the separation of strands within duplex nucleic acids to the physical remodeling or removal of nucleoprotein complexes. Here, we describe the characteristics of the Sf1a helicase Srs2 and the Sf1b helicase Pif1, both from the model organism Saccharomyces cerevisiae, focusing on the roles that they play in homologous recombination, a DNA repair pathway that is necessary for maintaining genome integrity. |
format | Online Article Text |
id | pubmed-8469786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84697862021-09-27 Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function Meir, Aviv Greene, Eric C. Genes (Basel) Review Helicases are enzymes that convert the chemical energy stored in ATP into mechanical work, allowing them to move along and manipulate nucleic acids. The helicase superfamily 1 (Sf1) is one of the largest subgroups of helicases and they are required for a range of cellular activities across all domains of life. Sf1 helicases can be further subdivided into two classes called the Sf1a and Sf1b helicases, which move in opposite directions on nucleic acids. The results of this movement can range from the separation of strands within duplex nucleic acids to the physical remodeling or removal of nucleoprotein complexes. Here, we describe the characteristics of the Sf1a helicase Srs2 and the Sf1b helicase Pif1, both from the model organism Saccharomyces cerevisiae, focusing on the roles that they play in homologous recombination, a DNA repair pathway that is necessary for maintaining genome integrity. MDPI 2021-08-26 /pmc/articles/PMC8469786/ /pubmed/34573298 http://dx.doi.org/10.3390/genes12091319 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Meir, Aviv Greene, Eric C. Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title | Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title_full | Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title_fullStr | Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title_full_unstemmed | Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title_short | Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function |
title_sort | srs2 and pif1 as model systems for understanding sf1a and sf1b helicase structure and function |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469786/ https://www.ncbi.nlm.nih.gov/pubmed/34573298 http://dx.doi.org/10.3390/genes12091319 |
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