Implementing Mass Rearing of Trissolcus japonicus (Hymenoptera: Scelionidae) on Cold-Stored Host Eggs

SIMPLE SUMMARY: Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) is a polyphagous and invasive pest that has been causing severe damage to agricultural systems worldwide including Italy. Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), an egg parasitoid native to regions of Asia where H. halys originated, seems to be one of the most promising solutions for decreasing pest pressure. In 2020, field releases of T. japonicus were approved in Italy with the aim of releasing 120,000 parasitoids. Thus, it was necessary to develop an efficient rearing system to support this biological control program. In this study, some parameters that could influence the rearing of T. japonicus on cold-stored eggs of H. halys were investigated. Refrigeration at 6 °C for as long as several months is an effective method of storage for H. halys egg masses. Female parasitoids produced the highest number of progeny when exposed to egg masses for 72 h. Adult wasps could also be stored at 16 °C for up to 90 days with a negligible decrease in survival, but a significant decrease in production of progeny. ABSTRACT: Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), a pest of Asian origin, has been causing severe damage to Italian agriculture. The application of classical biological control by the release of Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), an exotic egg parasitoid, appears to be one promising solution. In Italy, releases of T. japonicus in the field were authorized in 2020. In this study, some parameters that could influence the rearing of T. japonicus in insectaries were investigated. A significantly higher production of progeny was observed on host eggs stored at 6 °C (86.5%) compared to −24 °C (48.8%) for up to two months prior to exposure to parasitism. There were no significant differences in progeny production from single females in a vial provided with only one egg mass (83.2%) or 10 females inside a cage with 6 egg masses (83.9%). The exposure of parasitoids to refrigerated (6 °C) egg masses of H. halys for 72 h led to a significantly higher production of progeny (62.1%) compared to shorter exposures for 48 (44.0%) or 24 h (37.1%). A decline in production of progeny by the same female was detected between the first (62.1%) and the second parasitization (41.3%). Adult parasitoids stored at 16 °C for up to 90 days had an 87.1% survival rate, but a significant decrease in progeny production was detected. These parameters could be adjusted when rearing T. japonicus for specific aims such as the production of individuals for field release or colony maintenance.