Cargando…
Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems wou...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469811/ https://www.ncbi.nlm.nih.gov/pubmed/34577538 http://dx.doi.org/10.3390/ph14090838 |
_version_ | 1784574033585504256 |
---|---|
author | Kaminski, Kamil Skora, Magdalena Krzyściak, Paweł Stączek, Sylwia Zdybicka-Barabas, Agnieszka Cytryńska, Małgorzata |
author_facet | Kaminski, Kamil Skora, Magdalena Krzyściak, Paweł Stączek, Sylwia Zdybicka-Barabas, Agnieszka Cytryńska, Małgorzata |
author_sort | Kaminski, Kamil |
collection | PubMed |
description | The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems would have great potential in the treatment of infectious diseases, especially now when reports indicate the growing risk of fungal co-infections in COVID-19 patients. We describe in this article cationic derivatives of natural beta-glucan polymers obtained by reacting the polysaccharide isolated from Saccharomyces boulardii (SB) and Cetraria islandica (CI) with glycidyl trimethyl ammonium chloride (GTMAC). Two synthesis strategies were applied to optimize the product yield. Fungal diseases particularly affect low-income countries, hence the emphasis on the simplicity of the synthesis of such drugs so they can be produced without outside help. The three structures obtained showed selective anti-mycotic properties (against, i.e., Scopulariopsis brevicaulis, Aspergillus brasiliensis, and Fusarium solani), and their toxicity established using fibroblast 3T3-L1 cell line was negligible in a wide range of concentrations. For one of the polymers (SB derivative), using in vivo model of Aspergillus brasiliensis infection in Galleria mellonella insect model, we confirmed the promising results obtained in the preliminary study. |
format | Online Article Text |
id | pubmed-8469811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84698112021-09-27 Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives Kaminski, Kamil Skora, Magdalena Krzyściak, Paweł Stączek, Sylwia Zdybicka-Barabas, Agnieszka Cytryńska, Małgorzata Pharmaceuticals (Basel) Article The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems would have great potential in the treatment of infectious diseases, especially now when reports indicate the growing risk of fungal co-infections in COVID-19 patients. We describe in this article cationic derivatives of natural beta-glucan polymers obtained by reacting the polysaccharide isolated from Saccharomyces boulardii (SB) and Cetraria islandica (CI) with glycidyl trimethyl ammonium chloride (GTMAC). Two synthesis strategies were applied to optimize the product yield. Fungal diseases particularly affect low-income countries, hence the emphasis on the simplicity of the synthesis of such drugs so they can be produced without outside help. The three structures obtained showed selective anti-mycotic properties (against, i.e., Scopulariopsis brevicaulis, Aspergillus brasiliensis, and Fusarium solani), and their toxicity established using fibroblast 3T3-L1 cell line was negligible in a wide range of concentrations. For one of the polymers (SB derivative), using in vivo model of Aspergillus brasiliensis infection in Galleria mellonella insect model, we confirmed the promising results obtained in the preliminary study. MDPI 2021-08-24 /pmc/articles/PMC8469811/ /pubmed/34577538 http://dx.doi.org/10.3390/ph14090838 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kaminski, Kamil Skora, Magdalena Krzyściak, Paweł Stączek, Sylwia Zdybicka-Barabas, Agnieszka Cytryńska, Małgorzata Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title | Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title_full | Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title_fullStr | Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title_full_unstemmed | Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title_short | Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives |
title_sort | synthesis and study of antifungal properties of new cationic beta-glucan derivatives |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469811/ https://www.ncbi.nlm.nih.gov/pubmed/34577538 http://dx.doi.org/10.3390/ph14090838 |
work_keys_str_mv | AT kaminskikamil synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives AT skoramagdalena synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives AT krzysciakpaweł synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives AT staczeksylwia synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives AT zdybickabarabasagnieszka synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives AT cytrynskamałgorzata synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives |