Cargando…

Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives

The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems wou...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaminski, Kamil, Skora, Magdalena, Krzyściak, Paweł, Stączek, Sylwia, Zdybicka-Barabas, Agnieszka, Cytryńska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469811/
https://www.ncbi.nlm.nih.gov/pubmed/34577538
http://dx.doi.org/10.3390/ph14090838
_version_ 1784574033585504256
author Kaminski, Kamil
Skora, Magdalena
Krzyściak, Paweł
Stączek, Sylwia
Zdybicka-Barabas, Agnieszka
Cytryńska, Małgorzata
author_facet Kaminski, Kamil
Skora, Magdalena
Krzyściak, Paweł
Stączek, Sylwia
Zdybicka-Barabas, Agnieszka
Cytryńska, Małgorzata
author_sort Kaminski, Kamil
collection PubMed
description The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems would have great potential in the treatment of infectious diseases, especially now when reports indicate the growing risk of fungal co-infections in COVID-19 patients. We describe in this article cationic derivatives of natural beta-glucan polymers obtained by reacting the polysaccharide isolated from Saccharomyces boulardii (SB) and Cetraria islandica (CI) with glycidyl trimethyl ammonium chloride (GTMAC). Two synthesis strategies were applied to optimize the product yield. Fungal diseases particularly affect low-income countries, hence the emphasis on the simplicity of the synthesis of such drugs so they can be produced without outside help. The three structures obtained showed selective anti-mycotic properties (against, i.e., Scopulariopsis brevicaulis, Aspergillus brasiliensis, and Fusarium solani), and their toxicity established using fibroblast 3T3-L1 cell line was negligible in a wide range of concentrations. For one of the polymers (SB derivative), using in vivo model of Aspergillus brasiliensis infection in Galleria mellonella insect model, we confirmed the promising results obtained in the preliminary study.
format Online
Article
Text
id pubmed-8469811
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84698112021-09-27 Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives Kaminski, Kamil Skora, Magdalena Krzyściak, Paweł Stączek, Sylwia Zdybicka-Barabas, Agnieszka Cytryńska, Małgorzata Pharmaceuticals (Basel) Article The interaction of positively charged polymers (polycations) with a biological membrane is considered to be the cause of the frequently observed toxicity of these macromolecules. If it is possible to obtain polymers with a predominantly negative effect on bacterial and fungal cells, such systems would have great potential in the treatment of infectious diseases, especially now when reports indicate the growing risk of fungal co-infections in COVID-19 patients. We describe in this article cationic derivatives of natural beta-glucan polymers obtained by reacting the polysaccharide isolated from Saccharomyces boulardii (SB) and Cetraria islandica (CI) with glycidyl trimethyl ammonium chloride (GTMAC). Two synthesis strategies were applied to optimize the product yield. Fungal diseases particularly affect low-income countries, hence the emphasis on the simplicity of the synthesis of such drugs so they can be produced without outside help. The three structures obtained showed selective anti-mycotic properties (against, i.e., Scopulariopsis brevicaulis, Aspergillus brasiliensis, and Fusarium solani), and their toxicity established using fibroblast 3T3-L1 cell line was negligible in a wide range of concentrations. For one of the polymers (SB derivative), using in vivo model of Aspergillus brasiliensis infection in Galleria mellonella insect model, we confirmed the promising results obtained in the preliminary study. MDPI 2021-08-24 /pmc/articles/PMC8469811/ /pubmed/34577538 http://dx.doi.org/10.3390/ph14090838 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaminski, Kamil
Skora, Magdalena
Krzyściak, Paweł
Stączek, Sylwia
Zdybicka-Barabas, Agnieszka
Cytryńska, Małgorzata
Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title_full Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title_fullStr Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title_full_unstemmed Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title_short Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
title_sort synthesis and study of antifungal properties of new cationic beta-glucan derivatives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469811/
https://www.ncbi.nlm.nih.gov/pubmed/34577538
http://dx.doi.org/10.3390/ph14090838
work_keys_str_mv AT kaminskikamil synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives
AT skoramagdalena synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives
AT krzysciakpaweł synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives
AT staczeksylwia synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives
AT zdybickabarabasagnieszka synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives
AT cytrynskamałgorzata synthesisandstudyofantifungalpropertiesofnewcationicbetaglucanderivatives