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Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD

Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a d...

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Autores principales: Ancel, Julien, Belgacemi, Randa, Diabasana, Zania, Perotin, Jeanne-Marie, Bonnomet, Arnaud, Dewolf, Maxime, Launois, Claire, Mulette, Pauline, Deslée, Gaëtan, Polette, Myriam, Dormoy, Valérian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469815/
https://www.ncbi.nlm.nih.gov/pubmed/34573921
http://dx.doi.org/10.3390/diagnostics11091579
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author Ancel, Julien
Belgacemi, Randa
Diabasana, Zania
Perotin, Jeanne-Marie
Bonnomet, Arnaud
Dewolf, Maxime
Launois, Claire
Mulette, Pauline
Deslée, Gaëtan
Polette, Myriam
Dormoy, Valérian
author_facet Ancel, Julien
Belgacemi, Randa
Diabasana, Zania
Perotin, Jeanne-Marie
Bonnomet, Arnaud
Dewolf, Maxime
Launois, Claire
Mulette, Pauline
Deslée, Gaëtan
Polette, Myriam
Dormoy, Valérian
author_sort Ancel, Julien
collection PubMed
description Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD (n = 17) and non-COPD patients (n = 15). CBF was decreased by 30% in COPD (11.15 +/− 3.37 Hz vs. 7.89 +/− 3.39 Hz, p = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased (p < 0.005), the number of primary ciliated cells increased (p < 0.05) and primary cilia were shorter (p < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis.
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spelling pubmed-84698152021-09-27 Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD Ancel, Julien Belgacemi, Randa Diabasana, Zania Perotin, Jeanne-Marie Bonnomet, Arnaud Dewolf, Maxime Launois, Claire Mulette, Pauline Deslée, Gaëtan Polette, Myriam Dormoy, Valérian Diagnostics (Basel) Communication Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD (n = 17) and non-COPD patients (n = 15). CBF was decreased by 30% in COPD (11.15 +/− 3.37 Hz vs. 7.89 +/− 3.39 Hz, p = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased (p < 0.005), the number of primary ciliated cells increased (p < 0.05) and primary cilia were shorter (p < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis. MDPI 2021-08-31 /pmc/articles/PMC8469815/ /pubmed/34573921 http://dx.doi.org/10.3390/diagnostics11091579 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ancel, Julien
Belgacemi, Randa
Diabasana, Zania
Perotin, Jeanne-Marie
Bonnomet, Arnaud
Dewolf, Maxime
Launois, Claire
Mulette, Pauline
Deslée, Gaëtan
Polette, Myriam
Dormoy, Valérian
Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title_full Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title_fullStr Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title_full_unstemmed Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title_short Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
title_sort impaired ciliary beat frequency and ciliogenesis alteration during airway epithelial cell differentiation in copd
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469815/
https://www.ncbi.nlm.nih.gov/pubmed/34573921
http://dx.doi.org/10.3390/diagnostics11091579
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