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Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD
Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a d...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469815/ https://www.ncbi.nlm.nih.gov/pubmed/34573921 http://dx.doi.org/10.3390/diagnostics11091579 |
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author | Ancel, Julien Belgacemi, Randa Diabasana, Zania Perotin, Jeanne-Marie Bonnomet, Arnaud Dewolf, Maxime Launois, Claire Mulette, Pauline Deslée, Gaëtan Polette, Myriam Dormoy, Valérian |
author_facet | Ancel, Julien Belgacemi, Randa Diabasana, Zania Perotin, Jeanne-Marie Bonnomet, Arnaud Dewolf, Maxime Launois, Claire Mulette, Pauline Deslée, Gaëtan Polette, Myriam Dormoy, Valérian |
author_sort | Ancel, Julien |
collection | PubMed |
description | Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD (n = 17) and non-COPD patients (n = 15). CBF was decreased by 30% in COPD (11.15 +/− 3.37 Hz vs. 7.89 +/− 3.39 Hz, p = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased (p < 0.005), the number of primary ciliated cells increased (p < 0.05) and primary cilia were shorter (p < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis. |
format | Online Article Text |
id | pubmed-8469815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84698152021-09-27 Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD Ancel, Julien Belgacemi, Randa Diabasana, Zania Perotin, Jeanne-Marie Bonnomet, Arnaud Dewolf, Maxime Launois, Claire Mulette, Pauline Deslée, Gaëtan Polette, Myriam Dormoy, Valérian Diagnostics (Basel) Communication Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD (n = 17) and non-COPD patients (n = 15). CBF was decreased by 30% in COPD (11.15 +/− 3.37 Hz vs. 7.89 +/− 3.39 Hz, p = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased (p < 0.005), the number of primary ciliated cells increased (p < 0.05) and primary cilia were shorter (p < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis. MDPI 2021-08-31 /pmc/articles/PMC8469815/ /pubmed/34573921 http://dx.doi.org/10.3390/diagnostics11091579 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ancel, Julien Belgacemi, Randa Diabasana, Zania Perotin, Jeanne-Marie Bonnomet, Arnaud Dewolf, Maxime Launois, Claire Mulette, Pauline Deslée, Gaëtan Polette, Myriam Dormoy, Valérian Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title | Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title_full | Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title_fullStr | Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title_full_unstemmed | Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title_short | Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD |
title_sort | impaired ciliary beat frequency and ciliogenesis alteration during airway epithelial cell differentiation in copd |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469815/ https://www.ncbi.nlm.nih.gov/pubmed/34573921 http://dx.doi.org/10.3390/diagnostics11091579 |
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