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The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida

Heavy metal pollution is widespread and persistent, and causes serious harm to the environment. Pseudomonas putida, a representative environmental microorganism, has strong resistance to heavy metals due to its multiple efflux systems. Although the functions of many efflux systems have been well-stu...

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Autores principales: Liu, Huizhong, Zhang, Yu, Wang, Yingsi, Xie, Xiaobao, Shi, Qingshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469834/
https://www.ncbi.nlm.nih.gov/pubmed/34575861
http://dx.doi.org/10.3390/ijms22189697
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author Liu, Huizhong
Zhang, Yu
Wang, Yingsi
Xie, Xiaobao
Shi, Qingshan
author_facet Liu, Huizhong
Zhang, Yu
Wang, Yingsi
Xie, Xiaobao
Shi, Qingshan
author_sort Liu, Huizhong
collection PubMed
description Heavy metal pollution is widespread and persistent, and causes serious harm to the environment. Pseudomonas putida, a representative environmental microorganism, has strong resistance to heavy metals due to its multiple efflux systems. Although the functions of many efflux systems have been well-studied, the relationship between them remains unclear. Here, the relationship between the Czc and Cad systems that are predominantly responsible for cadmium efflux in P. putida KT2440 is identified. The results demonstrated that CzcR3, the response regulator of two-component system CzcRS3 in the Czc system, activates the expression of efflux pump genes czcCBA1 and czcCBA2 by directly binding to their promoters, thereby helping the strain resist cadmium stress. CzcR3 can also bind to its own promoter, but it has only a weak regulatory effect. The high-level expression of czcRS3 needs to be induced by Cd(2+), and this relies on the regulation of CadR, a key regulator in the Cad system, which showed affinity to czcRS3 promoter. Our study indicates that the Cad system is involved in the regulation of the Czc system, and this relationship is important for maintaining the considerable resistance to cadmium in P. putida.
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spelling pubmed-84698342021-09-27 The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida Liu, Huizhong Zhang, Yu Wang, Yingsi Xie, Xiaobao Shi, Qingshan Int J Mol Sci Article Heavy metal pollution is widespread and persistent, and causes serious harm to the environment. Pseudomonas putida, a representative environmental microorganism, has strong resistance to heavy metals due to its multiple efflux systems. Although the functions of many efflux systems have been well-studied, the relationship between them remains unclear. Here, the relationship between the Czc and Cad systems that are predominantly responsible for cadmium efflux in P. putida KT2440 is identified. The results demonstrated that CzcR3, the response regulator of two-component system CzcRS3 in the Czc system, activates the expression of efflux pump genes czcCBA1 and czcCBA2 by directly binding to their promoters, thereby helping the strain resist cadmium stress. CzcR3 can also bind to its own promoter, but it has only a weak regulatory effect. The high-level expression of czcRS3 needs to be induced by Cd(2+), and this relies on the regulation of CadR, a key regulator in the Cad system, which showed affinity to czcRS3 promoter. Our study indicates that the Cad system is involved in the regulation of the Czc system, and this relationship is important for maintaining the considerable resistance to cadmium in P. putida. MDPI 2021-09-08 /pmc/articles/PMC8469834/ /pubmed/34575861 http://dx.doi.org/10.3390/ijms22189697 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Huizhong
Zhang, Yu
Wang, Yingsi
Xie, Xiaobao
Shi, Qingshan
The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title_full The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title_fullStr The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title_full_unstemmed The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title_short The Connection between Czc and Cad Systems Involved in Cadmium Resistance in Pseudomonas putida
title_sort connection between czc and cad systems involved in cadmium resistance in pseudomonas putida
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469834/
https://www.ncbi.nlm.nih.gov/pubmed/34575861
http://dx.doi.org/10.3390/ijms22189697
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