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Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease

Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and...

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Autores principales: Lima, Gabriela de Morais Gouvêa, Borges, Aline Chiodi, Nishime, Thalita Mayumi Castaldelli, Santana-Melo, Gabriela de Fatima, Kostov, Konstantin Georgiev, Mayer, Marcia Pinto Alves, Koga-Ito, Cristiane Yumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470429/
https://www.ncbi.nlm.nih.gov/pubmed/34577061
http://dx.doi.org/10.3390/molecules26185590
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author Lima, Gabriela de Morais Gouvêa
Borges, Aline Chiodi
Nishime, Thalita Mayumi Castaldelli
Santana-Melo, Gabriela de Fatima
Kostov, Konstantin Georgiev
Mayer, Marcia Pinto Alves
Koga-Ito, Cristiane Yumi
author_facet Lima, Gabriela de Morais Gouvêa
Borges, Aline Chiodi
Nishime, Thalita Mayumi Castaldelli
Santana-Melo, Gabriela de Fatima
Kostov, Konstantin Georgiev
Mayer, Marcia Pinto Alves
Koga-Ito, Cristiane Yumi
author_sort Lima, Gabriela de Morais Gouvêa
collection PubMed
description Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.
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spelling pubmed-84704292021-09-27 Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease Lima, Gabriela de Morais Gouvêa Borges, Aline Chiodi Nishime, Thalita Mayumi Castaldelli Santana-Melo, Gabriela de Fatima Kostov, Konstantin Georgiev Mayer, Marcia Pinto Alves Koga-Ito, Cristiane Yumi Molecules Article Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications. MDPI 2021-09-15 /pmc/articles/PMC8470429/ /pubmed/34577061 http://dx.doi.org/10.3390/molecules26185590 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Gabriela de Morais Gouvêa
Borges, Aline Chiodi
Nishime, Thalita Mayumi Castaldelli
Santana-Melo, Gabriela de Fatima
Kostov, Konstantin Georgiev
Mayer, Marcia Pinto Alves
Koga-Ito, Cristiane Yumi
Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title_full Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title_fullStr Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title_full_unstemmed Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title_short Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
title_sort cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470429/
https://www.ncbi.nlm.nih.gov/pubmed/34577061
http://dx.doi.org/10.3390/molecules26185590
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