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Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease
Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470429/ https://www.ncbi.nlm.nih.gov/pubmed/34577061 http://dx.doi.org/10.3390/molecules26185590 |
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author | Lima, Gabriela de Morais Gouvêa Borges, Aline Chiodi Nishime, Thalita Mayumi Castaldelli Santana-Melo, Gabriela de Fatima Kostov, Konstantin Georgiev Mayer, Marcia Pinto Alves Koga-Ito, Cristiane Yumi |
author_facet | Lima, Gabriela de Morais Gouvêa Borges, Aline Chiodi Nishime, Thalita Mayumi Castaldelli Santana-Melo, Gabriela de Fatima Kostov, Konstantin Georgiev Mayer, Marcia Pinto Alves Koga-Ito, Cristiane Yumi |
author_sort | Lima, Gabriela de Morais Gouvêa |
collection | PubMed |
description | Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications. |
format | Online Article Text |
id | pubmed-8470429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84704292021-09-27 Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease Lima, Gabriela de Morais Gouvêa Borges, Aline Chiodi Nishime, Thalita Mayumi Castaldelli Santana-Melo, Gabriela de Fatima Kostov, Konstantin Georgiev Mayer, Marcia Pinto Alves Koga-Ito, Cristiane Yumi Molecules Article Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications. MDPI 2021-09-15 /pmc/articles/PMC8470429/ /pubmed/34577061 http://dx.doi.org/10.3390/molecules26185590 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima, Gabriela de Morais Gouvêa Borges, Aline Chiodi Nishime, Thalita Mayumi Castaldelli Santana-Melo, Gabriela de Fatima Kostov, Konstantin Georgiev Mayer, Marcia Pinto Alves Koga-Ito, Cristiane Yumi Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title | Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title_full | Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title_fullStr | Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title_full_unstemmed | Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title_short | Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease |
title_sort | cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470429/ https://www.ncbi.nlm.nih.gov/pubmed/34577061 http://dx.doi.org/10.3390/molecules26185590 |
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