Effects of Zinc Sources and Levels on Growth Performance, Zinc Status, Expressions of Zinc Transporters, and Zinc Bioavailability in Weaned Piglets

SIMPLE SUMMARY: Bioavailability of inorganic zinc in animals is low, and large amounts of zinc are excreted into feces, resulting in potential negative impacts on the environment and waste of zinc resources. To reduce zinc supplementation in animal feed, prepared and characterized chitosan–zinc (CS–Zn) chelate was studied to investigate its bioavailability. Dietary CS–Zn improved the weight gain of weaned piglets as compared to ZnSO(4). The Zn source had a significant influence on the liver, pancreas Zn contents, and the protein expression of ZnT1 and ZIP5 in duodenal mucosa. The Zn contents in the liver and pancreas and the protein expressions of ZnT1 and ZIP5 increased linearly with increases in the added Zn level. Multiple linear regression and the slope-ratio methodology showed that the bioavailability of CS–Zn was 110.9% or 149.0% relative to ZnSO(4), respectively, using zinc content in the liver or pancreas as the response parameter. These results indicate that CS–Zn shows enhanced bioavailability, suggesting a good potential substitute for inorganic zinc in animal nutrition. ABSTRACT: The present study was conducted to explore the bioavailability of chitosan–zinc chelate (CS–Zn) in weaned piglets, and its characteristics of prepared and oral safety were also involved. A total of 210 crossbred weaned piglets (Duroc × Landrace × Large White) with a mean body weight of 6.30 kg were randomly assigned into seven dietary treatments involving a 2 × 3 factorial arrangement with two Zn sources (CS–Zn and ZnSO(4)) and three levels of added Zn (50, 100, 150 mg Zn/kg) plus a Zn-unsupplemented control diet. The feeding trial lasted 42 days. The AFM image of CS–Zn showed a rougher appearance and smaller size particles. The changes in spectrum peaks evidenced the successful chelating of Zn(2+) with chitosan. The XRD patterns revealed the formation of a new crystalline phase. Moreover, the oral acute toxicity test of CS–Zn showed no lethal effects on mice. Weaned piglets fed dietary CS–Zn showed improved weight gain and decreased diarrhea incidence. Additionally, the bioavailability of CS–Zn was higher than that of ZnSO(4) in piglets. Taken together, these results indicate that the prepared CS–Zn chelate, with rough surface and crystalline phase, is non-toxic and show enhanced bioavailability.