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Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)

Of the six-glyphosate resistant weed species reported in Mexico, five were found in citrus groves. Here, the glyphosate susceptibility level and resistance mechanisms were evaluated in saltmarsh aster (Aster squamatus), a weed that also occurs in Mexican citrus groves. The R population accumulated 4...

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Autores principales: Domínguez-Valenzuela, José Alfredo, Alcántara-de la Cruz, Ricardo, Palma-Bautista, Candelario, Vázquez-García, José Guadalupe, Cruz-Hipolito, Hugo E., De Prado, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470777/
https://www.ncbi.nlm.nih.gov/pubmed/34579501
http://dx.doi.org/10.3390/plants10091970
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author Domínguez-Valenzuela, José Alfredo
Alcántara-de la Cruz, Ricardo
Palma-Bautista, Candelario
Vázquez-García, José Guadalupe
Cruz-Hipolito, Hugo E.
De Prado, Rafael
author_facet Domínguez-Valenzuela, José Alfredo
Alcántara-de la Cruz, Ricardo
Palma-Bautista, Candelario
Vázquez-García, José Guadalupe
Cruz-Hipolito, Hugo E.
De Prado, Rafael
author_sort Domínguez-Valenzuela, José Alfredo
collection PubMed
description Of the six-glyphosate resistant weed species reported in Mexico, five were found in citrus groves. Here, the glyphosate susceptibility level and resistance mechanisms were evaluated in saltmarsh aster (Aster squamatus), a weed that also occurs in Mexican citrus groves. The R population accumulated 4.5-fold less shikimic acid than S population. S plants hardly survived at 125 g ae ha(−1) while most of the R plants that were treated with 1000 g ae ha(−1), which suffered a strong growth arrest, showed a vigorous regrowth from the third week after treatment. Further, 5-enolpyruvylshikimate-3-phosphate basal and enzymatic activities did not diverge between populations, suggesting the absence of target-site resistance mechanisms. At 96 h after treatment, R plants absorbed ~18% less glyphosate and maintained 63% of the (14)C-glyphsoate absorbed in the treated leaf in comparison to S plants. R plants metabolized twice as much (72%) glyphosate to amino methyl phosphonic acid and glyoxylate as the S plants. Three non-target mechanisms, reduced absorption and translocation and increased metabolism, confer glyphosate resistance saltmarsh aster. This is the first case of glyphosate resistance recorded for A. squamatus in the world.
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spelling pubmed-84707772021-09-27 Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus) Domínguez-Valenzuela, José Alfredo Alcántara-de la Cruz, Ricardo Palma-Bautista, Candelario Vázquez-García, José Guadalupe Cruz-Hipolito, Hugo E. De Prado, Rafael Plants (Basel) Article Of the six-glyphosate resistant weed species reported in Mexico, five were found in citrus groves. Here, the glyphosate susceptibility level and resistance mechanisms were evaluated in saltmarsh aster (Aster squamatus), a weed that also occurs in Mexican citrus groves. The R population accumulated 4.5-fold less shikimic acid than S population. S plants hardly survived at 125 g ae ha(−1) while most of the R plants that were treated with 1000 g ae ha(−1), which suffered a strong growth arrest, showed a vigorous regrowth from the third week after treatment. Further, 5-enolpyruvylshikimate-3-phosphate basal and enzymatic activities did not diverge between populations, suggesting the absence of target-site resistance mechanisms. At 96 h after treatment, R plants absorbed ~18% less glyphosate and maintained 63% of the (14)C-glyphsoate absorbed in the treated leaf in comparison to S plants. R plants metabolized twice as much (72%) glyphosate to amino methyl phosphonic acid and glyoxylate as the S plants. Three non-target mechanisms, reduced absorption and translocation and increased metabolism, confer glyphosate resistance saltmarsh aster. This is the first case of glyphosate resistance recorded for A. squamatus in the world. MDPI 2021-09-21 /pmc/articles/PMC8470777/ /pubmed/34579501 http://dx.doi.org/10.3390/plants10091970 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Domínguez-Valenzuela, José Alfredo
Alcántara-de la Cruz, Ricardo
Palma-Bautista, Candelario
Vázquez-García, José Guadalupe
Cruz-Hipolito, Hugo E.
De Prado, Rafael
Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title_full Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title_fullStr Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title_full_unstemmed Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title_short Non-Target Site Mechanisms Endow Resistance to Glyphosate in Saltmarsh Aster (Aster squamatus)
title_sort non-target site mechanisms endow resistance to glyphosate in saltmarsh aster (aster squamatus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470777/
https://www.ncbi.nlm.nih.gov/pubmed/34579501
http://dx.doi.org/10.3390/plants10091970
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