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Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties
Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470853/ https://www.ncbi.nlm.nih.gov/pubmed/34576173 http://dx.doi.org/10.3390/ijms221810004 |
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author | Agregán-Pérez, Rubén Alonso-González, Elisa Mejuto, Juan Carlos Pérez-Guerra, Nelson |
author_facet | Agregán-Pérez, Rubén Alonso-González, Elisa Mejuto, Juan Carlos Pérez-Guerra, Nelson |
author_sort | Agregán-Pérez, Rubén |
collection | PubMed |
description | Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation of whey with a combination of kefir grains, AGK1, and the fermented whole milk used to activate these kefir grains. The probiotic culture obtained was characterized by high levels of biomass (8.03 g/L), total viability (3.6 × 10(8) CFU/mL) and antibacterial activity (28.26 Activity Units/mL). Some probiotic properties of the probiotic culture were investigated in vitro, including its survival at low pH values, under simulated gastrointestinal conditions, after freezing in skim milk at −20 °C, and in the commercial feed during storage at room temperature. The viable cells of lactic and acetic acid bacteria and yeasts exhibited higher tolerance to acidic pH and simulated gastrointestinal conditions when the cells were protected with skim milk and piglet feed, compared with washed cells. The results indicated the feasibility of producing a probiotic product at a low cost with a potential application in animal feed. |
format | Online Article Text |
id | pubmed-8470853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84708532021-09-27 Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties Agregán-Pérez, Rubén Alonso-González, Elisa Mejuto, Juan Carlos Pérez-Guerra, Nelson Int J Mol Sci Article Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation of whey with a combination of kefir grains, AGK1, and the fermented whole milk used to activate these kefir grains. The probiotic culture obtained was characterized by high levels of biomass (8.03 g/L), total viability (3.6 × 10(8) CFU/mL) and antibacterial activity (28.26 Activity Units/mL). Some probiotic properties of the probiotic culture were investigated in vitro, including its survival at low pH values, under simulated gastrointestinal conditions, after freezing in skim milk at −20 °C, and in the commercial feed during storage at room temperature. The viable cells of lactic and acetic acid bacteria and yeasts exhibited higher tolerance to acidic pH and simulated gastrointestinal conditions when the cells were protected with skim milk and piglet feed, compared with washed cells. The results indicated the feasibility of producing a probiotic product at a low cost with a potential application in animal feed. MDPI 2021-09-16 /pmc/articles/PMC8470853/ /pubmed/34576173 http://dx.doi.org/10.3390/ijms221810004 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Agregán-Pérez, Rubén Alonso-González, Elisa Mejuto, Juan Carlos Pérez-Guerra, Nelson Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title | Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title_full | Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title_fullStr | Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title_full_unstemmed | Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title_short | Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties |
title_sort | production of a potentially probiotic product for animal feed and evaluation of some of its probiotic properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470853/ https://www.ncbi.nlm.nih.gov/pubmed/34576173 http://dx.doi.org/10.3390/ijms221810004 |
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