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Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study

Several studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemi...

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Autores principales: Aguiar, Ana J. F. C., de Queiroz, Jaluza L. C., Santos, Pedro P. A., Camillo, Christina S., Serquiz, Alexandre C., Costa, Izael S., Oliveira, Gerciane S., Gomes, Ana F. T., Matias, Lídia L. R., Costa, Rafael O. A., Passos, Thaís S., Morais, Ana H. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470918/
https://www.ncbi.nlm.nih.gov/pubmed/34576130
http://dx.doi.org/10.3390/ijms22189968
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author Aguiar, Ana J. F. C.
de Queiroz, Jaluza L. C.
Santos, Pedro P. A.
Camillo, Christina S.
Serquiz, Alexandre C.
Costa, Izael S.
Oliveira, Gerciane S.
Gomes, Ana F. T.
Matias, Lídia L. R.
Costa, Rafael O. A.
Passos, Thaís S.
Morais, Ana H. A.
author_facet Aguiar, Ana J. F. C.
de Queiroz, Jaluza L. C.
Santos, Pedro P. A.
Camillo, Christina S.
Serquiz, Alexandre C.
Costa, Izael S.
Oliveira, Gerciane S.
Gomes, Ana F. T.
Matias, Lídia L. R.
Costa, Rafael O. A.
Passos, Thaís S.
Morais, Ana H. A.
author_sort Aguiar, Ana J. F. C.
collection PubMed
description Several studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemic index (HGLI) diet. The nanoformulations without TTI (CW) and ECW were obtained by nanoprecipitation technique, physically and chemically characterized, and then administered to the animals. The adult male Wistar rats (n = 20) were allocated to four groups: HGLI diet + water; standard diet + water; HGLI diet + ECW (12.5 mg/kg); and HGLI diet + CW (10.0 mg/kg), 1 mL per gagave, for ten days. They were evaluated using biochemical and hematological parameters, Fibrosis-4 Index for Liver Fibrosis (FIB-4), AST to Platelet Ratio Index (APRI) scores, and liver morphology. Both nanoparticles presented spherical shape, smooth surface, and nanometric size [120.7 nm (ECW) and 136.4 nm (CW)]. In animals, ECW reduced (p < 0.05) blood glucose (17%), glutamic oxalacetic transaminase (39%), and alkaline phosphatase (24%). Besides, ECW reduced (p < 0.05) APRI and FIB-4 scores and presented a better aspect of hepatic morphology. ECW promoted benefits over a liver injury caused by the HGLI diet.
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spelling pubmed-84709182021-09-27 Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study Aguiar, Ana J. F. C. de Queiroz, Jaluza L. C. Santos, Pedro P. A. Camillo, Christina S. Serquiz, Alexandre C. Costa, Izael S. Oliveira, Gerciane S. Gomes, Ana F. T. Matias, Lídia L. R. Costa, Rafael O. A. Passos, Thaís S. Morais, Ana H. A. Int J Mol Sci Article Several studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health status of the Wistar rats fed with a high glycemic index (HGLI) diet. The nanoformulations without TTI (CW) and ECW were obtained by nanoprecipitation technique, physically and chemically characterized, and then administered to the animals. The adult male Wistar rats (n = 20) were allocated to four groups: HGLI diet + water; standard diet + water; HGLI diet + ECW (12.5 mg/kg); and HGLI diet + CW (10.0 mg/kg), 1 mL per gagave, for ten days. They were evaluated using biochemical and hematological parameters, Fibrosis-4 Index for Liver Fibrosis (FIB-4), AST to Platelet Ratio Index (APRI) scores, and liver morphology. Both nanoparticles presented spherical shape, smooth surface, and nanometric size [120.7 nm (ECW) and 136.4 nm (CW)]. In animals, ECW reduced (p < 0.05) blood glucose (17%), glutamic oxalacetic transaminase (39%), and alkaline phosphatase (24%). Besides, ECW reduced (p < 0.05) APRI and FIB-4 scores and presented a better aspect of hepatic morphology. ECW promoted benefits over a liver injury caused by the HGLI diet. MDPI 2021-09-15 /pmc/articles/PMC8470918/ /pubmed/34576130 http://dx.doi.org/10.3390/ijms22189968 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aguiar, Ana J. F. C.
de Queiroz, Jaluza L. C.
Santos, Pedro P. A.
Camillo, Christina S.
Serquiz, Alexandre C.
Costa, Izael S.
Oliveira, Gerciane S.
Gomes, Ana F. T.
Matias, Lídia L. R.
Costa, Rafael O. A.
Passos, Thaís S.
Morais, Ana H. A.
Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title_full Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title_fullStr Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title_full_unstemmed Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title_short Beneficial Effects of Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles on Hepatic Injury Induced High Glycemic Index Diet: A Preclinical Study
title_sort beneficial effects of tamarind trypsin inhibitor in chitosan–whey protein nanoparticles on hepatic injury induced high glycemic index diet: a preclinical study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470918/
https://www.ncbi.nlm.nih.gov/pubmed/34576130
http://dx.doi.org/10.3390/ijms22189968
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