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Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core

Recent advances in G-quadruplex (GQ) studies have provided evidence for their important role in key biological processes (replication, transcription, genome stability, and epigenetics). These findings imply highly specific interactions between GQ structures and cellular proteins. The details of the...

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Detalles Bibliográficos
Autores principales: Svetlova, Julia, Sardushkin, Makar, Kolganova, Natalia, Timofeev, Edward
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471065/
https://www.ncbi.nlm.nih.gov/pubmed/34572544
http://dx.doi.org/10.3390/biom11091332
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author Svetlova, Julia
Sardushkin, Makar
Kolganova, Natalia
Timofeev, Edward
author_facet Svetlova, Julia
Sardushkin, Makar
Kolganova, Natalia
Timofeev, Edward
author_sort Svetlova, Julia
collection PubMed
description Recent advances in G-quadruplex (GQ) studies have provided evidence for their important role in key biological processes (replication, transcription, genome stability, and epigenetics). These findings imply highly specific interactions between GQ structures and cellular proteins. The details of the interaction between GQs and cellular proteins remain unknown. It is now accepted that GQ loop elements play a major role in protein recognition. It remains unclear whether and to what extent the GQ core contributes to maintaining the recognition interface. In the current paper, we used the thrombin binding aptamer as a model to study the effect of modification in the quadruplex core on the ability of aptamer to interact with thrombin. We used alpha-2′-deoxyguanosine and 8-bromo-2′-deoxyguanosine to reconfigure the core or to affect syn–anti preferences of selected dG-residues. Our data suggest that core guanines not only support a particular type of GQ architecture, but also set structural parameters that make GQ protein recognition sensitive to quadruplex topology.
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spelling pubmed-84710652021-09-27 Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core Svetlova, Julia Sardushkin, Makar Kolganova, Natalia Timofeev, Edward Biomolecules Article Recent advances in G-quadruplex (GQ) studies have provided evidence for their important role in key biological processes (replication, transcription, genome stability, and epigenetics). These findings imply highly specific interactions between GQ structures and cellular proteins. The details of the interaction between GQs and cellular proteins remain unknown. It is now accepted that GQ loop elements play a major role in protein recognition. It remains unclear whether and to what extent the GQ core contributes to maintaining the recognition interface. In the current paper, we used the thrombin binding aptamer as a model to study the effect of modification in the quadruplex core on the ability of aptamer to interact with thrombin. We used alpha-2′-deoxyguanosine and 8-bromo-2′-deoxyguanosine to reconfigure the core or to affect syn–anti preferences of selected dG-residues. Our data suggest that core guanines not only support a particular type of GQ architecture, but also set structural parameters that make GQ protein recognition sensitive to quadruplex topology. MDPI 2021-09-09 /pmc/articles/PMC8471065/ /pubmed/34572544 http://dx.doi.org/10.3390/biom11091332 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Svetlova, Julia
Sardushkin, Makar
Kolganova, Natalia
Timofeev, Edward
Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title_full Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title_fullStr Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title_full_unstemmed Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title_short Recognition Interface of the Thrombin Binding Aptamer Requires Antiparallel Topology of the Quadruplex Core
title_sort recognition interface of the thrombin binding aptamer requires antiparallel topology of the quadruplex core
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471065/
https://www.ncbi.nlm.nih.gov/pubmed/34572544
http://dx.doi.org/10.3390/biom11091332
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