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A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use

The excessive use of antibiotics in both human and veterinary medicine has contributed to the development and rapid spread of drug resistance in bacteria. Silver nanoparticles (AgNPs) have become a tool of choice that can be used to treat these resistant bacteria. Several studies have shown that AgN...

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Autores principales: Thammawithan, Saengrawee, Siritongsuk, Pawinee, Nasompag, Sawinee, Daduang, Sakda, Klaynongsruang, Sompong, Prapasarakul, Nuvee, Patramanon, Rina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471216/
https://www.ncbi.nlm.nih.gov/pubmed/34564571
http://dx.doi.org/10.3390/vetsci8090177
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author Thammawithan, Saengrawee
Siritongsuk, Pawinee
Nasompag, Sawinee
Daduang, Sakda
Klaynongsruang, Sompong
Prapasarakul, Nuvee
Patramanon, Rina
author_facet Thammawithan, Saengrawee
Siritongsuk, Pawinee
Nasompag, Sawinee
Daduang, Sakda
Klaynongsruang, Sompong
Prapasarakul, Nuvee
Patramanon, Rina
author_sort Thammawithan, Saengrawee
collection PubMed
description The excessive use of antibiotics in both human and veterinary medicine has contributed to the development and rapid spread of drug resistance in bacteria. Silver nanoparticles (AgNPs) have become a tool of choice that can be used to treat these resistant bacteria. Several studies have shown that AgNPs have antibacterial and wound healing properties. In this study, we evaluated the biological activity of anisotropic AgNPs to develop an antimicrobial gel formulation for treating wound infections. We showed that some anisotropic AgNPs (S2) have an effective antibacterial activity against bacterial pathogens and low cytotoxicity to keratinocytes and fibroblasts in vitro. The MIC and MBC values were in the range of 2–32 µg/mL, and cytotoxicity had IC(50) values of 68.20 ± 9.71 µg/mL and 68.65 ± 10.97 µg/mL against human keratinocyte and normal human dermal fibroblast cells, respectively. The anisotropic AgNPs (S2) were used as a gel component and tested for antibacterial activity, including long-term protection, compared with povidone iodine, a common antiseptic agent. The results show that the anisotropic AgNPs can inhibit the growth of most tested bacterial pathogens and provide protection longer than 48 h, whereas povidone iodine only inhibits the growth of some bacteria. This study suggests that anisotropic AgNPs could be used as an alternative antimicrobial agent for treating bacterial skin infection and as a wound healing formulation.
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spelling pubmed-84712162021-09-27 A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use Thammawithan, Saengrawee Siritongsuk, Pawinee Nasompag, Sawinee Daduang, Sakda Klaynongsruang, Sompong Prapasarakul, Nuvee Patramanon, Rina Vet Sci Article The excessive use of antibiotics in both human and veterinary medicine has contributed to the development and rapid spread of drug resistance in bacteria. Silver nanoparticles (AgNPs) have become a tool of choice that can be used to treat these resistant bacteria. Several studies have shown that AgNPs have antibacterial and wound healing properties. In this study, we evaluated the biological activity of anisotropic AgNPs to develop an antimicrobial gel formulation for treating wound infections. We showed that some anisotropic AgNPs (S2) have an effective antibacterial activity against bacterial pathogens and low cytotoxicity to keratinocytes and fibroblasts in vitro. The MIC and MBC values were in the range of 2–32 µg/mL, and cytotoxicity had IC(50) values of 68.20 ± 9.71 µg/mL and 68.65 ± 10.97 µg/mL against human keratinocyte and normal human dermal fibroblast cells, respectively. The anisotropic AgNPs (S2) were used as a gel component and tested for antibacterial activity, including long-term protection, compared with povidone iodine, a common antiseptic agent. The results show that the anisotropic AgNPs can inhibit the growth of most tested bacterial pathogens and provide protection longer than 48 h, whereas povidone iodine only inhibits the growth of some bacteria. This study suggests that anisotropic AgNPs could be used as an alternative antimicrobial agent for treating bacterial skin infection and as a wound healing formulation. MDPI 2021-08-31 /pmc/articles/PMC8471216/ /pubmed/34564571 http://dx.doi.org/10.3390/vetsci8090177 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thammawithan, Saengrawee
Siritongsuk, Pawinee
Nasompag, Sawinee
Daduang, Sakda
Klaynongsruang, Sompong
Prapasarakul, Nuvee
Patramanon, Rina
A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title_full A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title_fullStr A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title_full_unstemmed A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title_short A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use
title_sort biological study of anisotropic silver nanoparticles and their antimicrobial application for topical use
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471216/
https://www.ncbi.nlm.nih.gov/pubmed/34564571
http://dx.doi.org/10.3390/vetsci8090177
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