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Radiolabeling Strategies of Nanobodies for Imaging Applications

Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, w...

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Autores principales: Küppers, Jim, Kürpig, Stefan, Bundschuh, Ralph A., Essler, Markus, Lütje, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471529/
https://www.ncbi.nlm.nih.gov/pubmed/34573872
http://dx.doi.org/10.3390/diagnostics11091530
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author Küppers, Jim
Kürpig, Stefan
Bundschuh, Ralph A.
Essler, Markus
Lütje, Susanne
author_facet Küppers, Jim
Kürpig, Stefan
Bundschuh, Ralph A.
Essler, Markus
Lütje, Susanne
author_sort Küppers, Jim
collection PubMed
description Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, while unbound molecules are quickly cleared from the circulation. In consequence, high signal-to-background ratios can be achieved, rendering radiolabeled nanobodies high-potential candidates for imaging applications in oncology, immunology and specific diseases, for instance in the cardiovascular system. In this review, a comprehensive overview of central aspects of nanobody functionalization and radiolabeling strategies is provided.
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spelling pubmed-84715292021-09-28 Radiolabeling Strategies of Nanobodies for Imaging Applications Küppers, Jim Kürpig, Stefan Bundschuh, Ralph A. Essler, Markus Lütje, Susanne Diagnostics (Basel) Review Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, while unbound molecules are quickly cleared from the circulation. In consequence, high signal-to-background ratios can be achieved, rendering radiolabeled nanobodies high-potential candidates for imaging applications in oncology, immunology and specific diseases, for instance in the cardiovascular system. In this review, a comprehensive overview of central aspects of nanobody functionalization and radiolabeling strategies is provided. MDPI 2021-08-25 /pmc/articles/PMC8471529/ /pubmed/34573872 http://dx.doi.org/10.3390/diagnostics11091530 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Küppers, Jim
Kürpig, Stefan
Bundschuh, Ralph A.
Essler, Markus
Lütje, Susanne
Radiolabeling Strategies of Nanobodies for Imaging Applications
title Radiolabeling Strategies of Nanobodies for Imaging Applications
title_full Radiolabeling Strategies of Nanobodies for Imaging Applications
title_fullStr Radiolabeling Strategies of Nanobodies for Imaging Applications
title_full_unstemmed Radiolabeling Strategies of Nanobodies for Imaging Applications
title_short Radiolabeling Strategies of Nanobodies for Imaging Applications
title_sort radiolabeling strategies of nanobodies for imaging applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471529/
https://www.ncbi.nlm.nih.gov/pubmed/34573872
http://dx.doi.org/10.3390/diagnostics11091530
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