Cargando…
Radiolabeling Strategies of Nanobodies for Imaging Applications
Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, w...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471529/ https://www.ncbi.nlm.nih.gov/pubmed/34573872 http://dx.doi.org/10.3390/diagnostics11091530 |
_version_ | 1784574490399735808 |
---|---|
author | Küppers, Jim Kürpig, Stefan Bundschuh, Ralph A. Essler, Markus Lütje, Susanne |
author_facet | Küppers, Jim Kürpig, Stefan Bundschuh, Ralph A. Essler, Markus Lütje, Susanne |
author_sort | Küppers, Jim |
collection | PubMed |
description | Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, while unbound molecules are quickly cleared from the circulation. In consequence, high signal-to-background ratios can be achieved, rendering radiolabeled nanobodies high-potential candidates for imaging applications in oncology, immunology and specific diseases, for instance in the cardiovascular system. In this review, a comprehensive overview of central aspects of nanobody functionalization and radiolabeling strategies is provided. |
format | Online Article Text |
id | pubmed-8471529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84715292021-09-28 Radiolabeling Strategies of Nanobodies for Imaging Applications Küppers, Jim Kürpig, Stefan Bundschuh, Ralph A. Essler, Markus Lütje, Susanne Diagnostics (Basel) Review Nanobodies are small recombinant antigen-binding fragments derived from camelid heavy-chain only antibodies. Due to their compact structure, pharmacokinetics of nanobodies are favorable compared to full-size antibodies, allowing rapid accumulation to their targets after intravenous administration, while unbound molecules are quickly cleared from the circulation. In consequence, high signal-to-background ratios can be achieved, rendering radiolabeled nanobodies high-potential candidates for imaging applications in oncology, immunology and specific diseases, for instance in the cardiovascular system. In this review, a comprehensive overview of central aspects of nanobody functionalization and radiolabeling strategies is provided. MDPI 2021-08-25 /pmc/articles/PMC8471529/ /pubmed/34573872 http://dx.doi.org/10.3390/diagnostics11091530 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Küppers, Jim Kürpig, Stefan Bundschuh, Ralph A. Essler, Markus Lütje, Susanne Radiolabeling Strategies of Nanobodies for Imaging Applications |
title | Radiolabeling Strategies of Nanobodies for Imaging Applications |
title_full | Radiolabeling Strategies of Nanobodies for Imaging Applications |
title_fullStr | Radiolabeling Strategies of Nanobodies for Imaging Applications |
title_full_unstemmed | Radiolabeling Strategies of Nanobodies for Imaging Applications |
title_short | Radiolabeling Strategies of Nanobodies for Imaging Applications |
title_sort | radiolabeling strategies of nanobodies for imaging applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471529/ https://www.ncbi.nlm.nih.gov/pubmed/34573872 http://dx.doi.org/10.3390/diagnostics11091530 |
work_keys_str_mv | AT kuppersjim radiolabelingstrategiesofnanobodiesforimagingapplications AT kurpigstefan radiolabelingstrategiesofnanobodiesforimagingapplications AT bundschuhralpha radiolabelingstrategiesofnanobodiesforimagingapplications AT esslermarkus radiolabelingstrategiesofnanobodiesforimagingapplications AT lutjesusanne radiolabelingstrategiesofnanobodiesforimagingapplications |