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Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes

Human rhinovirus (RV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from RV A and 8 from RV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in RV A and C serotypes and pr...

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Autores principales: Gomez-Perosanz, Marta, Fiyouzi, Tara, Fernandez-Arquero, Miguel, Sidney, John, Sette, Alessandro, Reinherz, Ellis L., Lafuente, Esther M., Reche, Pedro A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471592/
https://www.ncbi.nlm.nih.gov/pubmed/34571943
http://dx.doi.org/10.3390/cells10092294
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author Gomez-Perosanz, Marta
Fiyouzi, Tara
Fernandez-Arquero, Miguel
Sidney, John
Sette, Alessandro
Reinherz, Ellis L.
Lafuente, Esther M.
Reche, Pedro A.
author_facet Gomez-Perosanz, Marta
Fiyouzi, Tara
Fernandez-Arquero, Miguel
Sidney, John
Sette, Alessandro
Reinherz, Ellis L.
Lafuente, Esther M.
Reche, Pedro A.
author_sort Gomez-Perosanz, Marta
collection PubMed
description Human rhinovirus (RV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from RV A and 8 from RV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in RV A and C serotypes and predicted to bind to multiple human leukocyte antigen class II (HLA II) molecules. We found positive T cell recall responses by interferon gamma (IFNγ)-ELISPOT assays to eight peptides, validating seven of them (three from RV A and four from RV C) as CD4 T cell epitopes through intracellular cytokine staining assays. Additionally, we verified their promiscuous binding to multiple HLA II molecules by quantitative binding assays. According to their experimental HLA II binding profile, the combination of all these seven epitopes could be recognized by >95% of the world population. We actually determined IFNγ responses to a pool encompassing these CD4 T cell epitopes by intracellular cytokine staining, finding positive responses in 29 out of 30 donors. The CD4 T cell epitopes identified in this study could be key to monitor RV infections and to develop peptide-based vaccines against most RV A and C serotypes.
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spelling pubmed-84715922021-09-28 Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes Gomez-Perosanz, Marta Fiyouzi, Tara Fernandez-Arquero, Miguel Sidney, John Sette, Alessandro Reinherz, Ellis L. Lafuente, Esther M. Reche, Pedro A. Cells Article Human rhinovirus (RV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from RV A and 8 from RV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in RV A and C serotypes and predicted to bind to multiple human leukocyte antigen class II (HLA II) molecules. We found positive T cell recall responses by interferon gamma (IFNγ)-ELISPOT assays to eight peptides, validating seven of them (three from RV A and four from RV C) as CD4 T cell epitopes through intracellular cytokine staining assays. Additionally, we verified their promiscuous binding to multiple HLA II molecules by quantitative binding assays. According to their experimental HLA II binding profile, the combination of all these seven epitopes could be recognized by >95% of the world population. We actually determined IFNγ responses to a pool encompassing these CD4 T cell epitopes by intracellular cytokine staining, finding positive responses in 29 out of 30 donors. The CD4 T cell epitopes identified in this study could be key to monitor RV infections and to develop peptide-based vaccines against most RV A and C serotypes. MDPI 2021-09-02 /pmc/articles/PMC8471592/ /pubmed/34571943 http://dx.doi.org/10.3390/cells10092294 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gomez-Perosanz, Marta
Fiyouzi, Tara
Fernandez-Arquero, Miguel
Sidney, John
Sette, Alessandro
Reinherz, Ellis L.
Lafuente, Esther M.
Reche, Pedro A.
Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title_full Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title_fullStr Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title_full_unstemmed Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title_short Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes
title_sort characterization of conserved and promiscuous human rhinovirus cd4 t cell epitopes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471592/
https://www.ncbi.nlm.nih.gov/pubmed/34571943
http://dx.doi.org/10.3390/cells10092294
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