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Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure
Myocardial infarction (MI) remains the leading cause of cardiovascular death worldwide and a major cause of heart failure. Recent studies have suggested that cell-based therapies with bone marrow stem cells (BMSC) and human amniotic membrane (hAM) would recover the ventricular function after MI; how...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471644/ https://www.ncbi.nlm.nih.gov/pubmed/34575107 http://dx.doi.org/10.3390/life11090958 |
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author | Blume, Gustavo Gavazzoni Machado-Junior, Paulo André Bispo Simeoni, Rossana Baggio Bertinato, Giovana Paludo Tonial, Murilo Sgarbossa Nagashima, Seigo Pinho, Ricardo Aurino de Noronha, Lucia Olandoski, Marcia de Carvalho, Katherine Athayde Teixeira Francisco, Julio Cesar Guarita-Souza, Luiz Cesar |
author_facet | Blume, Gustavo Gavazzoni Machado-Junior, Paulo André Bispo Simeoni, Rossana Baggio Bertinato, Giovana Paludo Tonial, Murilo Sgarbossa Nagashima, Seigo Pinho, Ricardo Aurino de Noronha, Lucia Olandoski, Marcia de Carvalho, Katherine Athayde Teixeira Francisco, Julio Cesar Guarita-Souza, Luiz Cesar |
author_sort | Blume, Gustavo Gavazzoni |
collection | PubMed |
description | Myocardial infarction (MI) remains the leading cause of cardiovascular death worldwide and a major cause of heart failure. Recent studies have suggested that cell-based therapies with bone marrow stem cells (BMSC) and human amniotic membrane (hAM) would recover the ventricular function after MI; however, the mechanisms underlying these effects are still controversial. Herein, we aimed to compare the effects of BMSC and hAM in a rat model of heart failure. MI was induced through coronary occlusion, and animals with an ejection fraction (EF) < 50% were included and randomized into three groups: control, BMSC, and hAM. The BMSC and hAM groups were implanted on the anterior ventricular wall seven days after MI, and a new echocardiographic analysis was performed on the 30th day, followed by euthanasia. The echocardiographic results after 30 days showed significant improvements on EF and left-ventricular end-sistolic and end-diastolic volumes in both BMSC and hAM groups, without significant benefits in the control group. New blood vessels, desmine-positive cells and connexin-43 expression were also elevated in both BMSC and hAM groups. These results suggest a recovery of global cardiac function with the therapeutic use of both BMSC and hAM, associated with angiogenesis and cardiomyocyte regeneration after 30 days. |
format | Online Article Text |
id | pubmed-8471644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84716442021-09-28 Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure Blume, Gustavo Gavazzoni Machado-Junior, Paulo André Bispo Simeoni, Rossana Baggio Bertinato, Giovana Paludo Tonial, Murilo Sgarbossa Nagashima, Seigo Pinho, Ricardo Aurino de Noronha, Lucia Olandoski, Marcia de Carvalho, Katherine Athayde Teixeira Francisco, Julio Cesar Guarita-Souza, Luiz Cesar Life (Basel) Article Myocardial infarction (MI) remains the leading cause of cardiovascular death worldwide and a major cause of heart failure. Recent studies have suggested that cell-based therapies with bone marrow stem cells (BMSC) and human amniotic membrane (hAM) would recover the ventricular function after MI; however, the mechanisms underlying these effects are still controversial. Herein, we aimed to compare the effects of BMSC and hAM in a rat model of heart failure. MI was induced through coronary occlusion, and animals with an ejection fraction (EF) < 50% were included and randomized into three groups: control, BMSC, and hAM. The BMSC and hAM groups were implanted on the anterior ventricular wall seven days after MI, and a new echocardiographic analysis was performed on the 30th day, followed by euthanasia. The echocardiographic results after 30 days showed significant improvements on EF and left-ventricular end-sistolic and end-diastolic volumes in both BMSC and hAM groups, without significant benefits in the control group. New blood vessels, desmine-positive cells and connexin-43 expression were also elevated in both BMSC and hAM groups. These results suggest a recovery of global cardiac function with the therapeutic use of both BMSC and hAM, associated with angiogenesis and cardiomyocyte regeneration after 30 days. MDPI 2021-09-13 /pmc/articles/PMC8471644/ /pubmed/34575107 http://dx.doi.org/10.3390/life11090958 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Blume, Gustavo Gavazzoni Machado-Junior, Paulo André Bispo Simeoni, Rossana Baggio Bertinato, Giovana Paludo Tonial, Murilo Sgarbossa Nagashima, Seigo Pinho, Ricardo Aurino de Noronha, Lucia Olandoski, Marcia de Carvalho, Katherine Athayde Teixeira Francisco, Julio Cesar Guarita-Souza, Luiz Cesar Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title | Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title_full | Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title_fullStr | Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title_full_unstemmed | Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title_short | Bone-Marrow Stem Cells and Acellular Human Amniotic Membrane in a Rat Model of Heart Failure |
title_sort | bone-marrow stem cells and acellular human amniotic membrane in a rat model of heart failure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471644/ https://www.ncbi.nlm.nih.gov/pubmed/34575107 http://dx.doi.org/10.3390/life11090958 |
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