A Longitudinal Study of Hematology and Stress Biomarker Profiles in Young Asian Elephants (Elephas Maximus) in Relation to Elephant Endotheliotropic Herpesvirus (EEHV) in Thailand

SIMPLE SUMMARY: A change in hematology profiles is one indicator of EEHV infection before clinical signs appear; however, to be effective, individual baselines and age-matched reference values are needed. A longitudinal investigation of viremia, hematology values, and stress biomarkers was performed in three non-EEHV and six prior infected EEHV calves to better understand EEHV-HD-associated factors. Blood, saliva, and feces were collected for 1 year for analysis of complete blood count (CBC), viral load, glucocorticoids (GCs), and Immunoglobulin A (IgA). Results did not differ between the groups, except for one elephant that presented with EEHV-HD during the study and exhibited high viremia, altered hematology profiles, and decreased stress biomarker concentrations. Thus, as in other studies, hematology changes were associated with EEHV infection, while preliminary data in one calf suggests that stress-response measures might also be informative and warrant further investigation. ABSTRACT: Elephant endotheliotropic herpesvirus hemorrhagic disease (EEHV-HD) is a virulent disease that causes severe hemorrhage and sudden death in Asian elephant calves. A change in hematology profiles is one indicator of infection before clinical signs appear; however, to be effective, individual baselines and age-matched reference values are needed. Stress has been speculated to be a factor in clinical EEHV cases, but relationships have not been demonstrated empirically. This study evaluated blood hematology and several stress response markers—salivary cortisol, fecal glucocorticoid metabolites (FGM), salivary Immunoglobulin A (SIgA), and fecal IgA (FIgA) in samples collected for 1 year from three healthy calves with no EEHV history (non-EEHV), and six that had previously been infected, developed clinical signs and survived (prior-EEHV). Hematology values between non-EEHV and prior-EEHV elephants were not different and within published reference ranges. Concentrations of salivary cortisol, FGM, SIgA, and FIgA also were variable and showed seasonal differences, but no relationships to prior EEHV status. One of the prior EEHV calves became re-infected, developed hemorrhagic disease (HD), and died during the study period. That calf exhibited lymphocytopenia, monocytopenia, and thrombocytopenia. Additionally, all stress biomarker concentrations were lower in the 12 days before viremia was observed. Thus, as in other studies, changes in hematology occur with EEHV infection, while preliminary data in one calf suggests that stress-response measures might also be informative and should be studied further.