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Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress

Oxidative stress is a known contributor to the progression of dry eye disease pathophysiology, and previous studies have shown that antioxidant intervention is a promising therapeutic approach to reduce the disease burden and slow disease progression. In this study, we evaluated the pharmacological...

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Autores principales: Ghosh, Anita Kirti, Thapa, Rubina, Hariani, Harsh Nilesh, Volyanyuk, Michael, Ogle, Sean David, Orloff, Karoline Anne, Ankireddy, Samatha, Lai, Karen, Žiniauskaitė, Agnė, Stubbs, Evan Benjamin, Kalesnykas, Giedrius, Hakkarainen, Jenni Johanna, Langert, Kelly Ann, Kaja, Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471707/
https://www.ncbi.nlm.nih.gov/pubmed/34575438
http://dx.doi.org/10.3390/pharmaceutics13091362
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author Ghosh, Anita Kirti
Thapa, Rubina
Hariani, Harsh Nilesh
Volyanyuk, Michael
Ogle, Sean David
Orloff, Karoline Anne
Ankireddy, Samatha
Lai, Karen
Žiniauskaitė, Agnė
Stubbs, Evan Benjamin
Kalesnykas, Giedrius
Hakkarainen, Jenni Johanna
Langert, Kelly Ann
Kaja, Simon
author_facet Ghosh, Anita Kirti
Thapa, Rubina
Hariani, Harsh Nilesh
Volyanyuk, Michael
Ogle, Sean David
Orloff, Karoline Anne
Ankireddy, Samatha
Lai, Karen
Žiniauskaitė, Agnė
Stubbs, Evan Benjamin
Kalesnykas, Giedrius
Hakkarainen, Jenni Johanna
Langert, Kelly Ann
Kaja, Simon
author_sort Ghosh, Anita Kirti
collection PubMed
description Oxidative stress is a known contributor to the progression of dry eye disease pathophysiology, and previous studies have shown that antioxidant intervention is a promising therapeutic approach to reduce the disease burden and slow disease progression. In this study, we evaluated the pharmacological efficacy of the naturally occurring prenylated chalconoid, xanthohumol, in preclinical models for dry eye disease. Xanthohumol acts by promoting the transcription of phase II antioxidant enzymes. In this study, xanthohumol prevented tert-butyl hydroperoxide-induced loss of cell viability in human corneal epithelial (HCE-T) cells in a dose-dependent manner and resulted in a significant increase in expression of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), the master regulator of phase II endogenous antioxidant enzymes. Xanthohumol-encapsulating poly(lactic-co-glycolic acid) nanoparticles (PLGA NP) were cytoprotective against oxidative stress in vitro, and significantly reduced ocular surface damage and oxidative stress-associated DNA damage in corneal epithelial cells in the mouse desiccating stress/scopolamine model for dry eye disease in vivo. PLGA NP represent a safe and efficacious drug delivery vehicle for hydrophobic small molecules to the ocular surface. Optimization of NP-based antioxidant formulations with the goal to minimize instillation frequency may represent future therapeutic options for dry eye disease and related ocular surface disease.
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spelling pubmed-84717072021-09-28 Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress Ghosh, Anita Kirti Thapa, Rubina Hariani, Harsh Nilesh Volyanyuk, Michael Ogle, Sean David Orloff, Karoline Anne Ankireddy, Samatha Lai, Karen Žiniauskaitė, Agnė Stubbs, Evan Benjamin Kalesnykas, Giedrius Hakkarainen, Jenni Johanna Langert, Kelly Ann Kaja, Simon Pharmaceutics Article Oxidative stress is a known contributor to the progression of dry eye disease pathophysiology, and previous studies have shown that antioxidant intervention is a promising therapeutic approach to reduce the disease burden and slow disease progression. In this study, we evaluated the pharmacological efficacy of the naturally occurring prenylated chalconoid, xanthohumol, in preclinical models for dry eye disease. Xanthohumol acts by promoting the transcription of phase II antioxidant enzymes. In this study, xanthohumol prevented tert-butyl hydroperoxide-induced loss of cell viability in human corneal epithelial (HCE-T) cells in a dose-dependent manner and resulted in a significant increase in expression of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2), the master regulator of phase II endogenous antioxidant enzymes. Xanthohumol-encapsulating poly(lactic-co-glycolic acid) nanoparticles (PLGA NP) were cytoprotective against oxidative stress in vitro, and significantly reduced ocular surface damage and oxidative stress-associated DNA damage in corneal epithelial cells in the mouse desiccating stress/scopolamine model for dry eye disease in vivo. PLGA NP represent a safe and efficacious drug delivery vehicle for hydrophobic small molecules to the ocular surface. Optimization of NP-based antioxidant formulations with the goal to minimize instillation frequency may represent future therapeutic options for dry eye disease and related ocular surface disease. MDPI 2021-08-30 /pmc/articles/PMC8471707/ /pubmed/34575438 http://dx.doi.org/10.3390/pharmaceutics13091362 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ghosh, Anita Kirti
Thapa, Rubina
Hariani, Harsh Nilesh
Volyanyuk, Michael
Ogle, Sean David
Orloff, Karoline Anne
Ankireddy, Samatha
Lai, Karen
Žiniauskaitė, Agnė
Stubbs, Evan Benjamin
Kalesnykas, Giedrius
Hakkarainen, Jenni Johanna
Langert, Kelly Ann
Kaja, Simon
Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title_full Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title_fullStr Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title_full_unstemmed Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title_short Poly(lactic-co-glycolic acid) Nanoparticles Encapsulating the Prenylated Flavonoid, Xanthohumol, Protect Corneal Epithelial Cells from Dry Eye Disease-Associated Oxidative Stress
title_sort poly(lactic-co-glycolic acid) nanoparticles encapsulating the prenylated flavonoid, xanthohumol, protect corneal epithelial cells from dry eye disease-associated oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471707/
https://www.ncbi.nlm.nih.gov/pubmed/34575438
http://dx.doi.org/10.3390/pharmaceutics13091362
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