Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature

SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of the storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C for up to 9 days had no significant effect, while storage at −20 °C led to a significant reduction in gene copy...

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Autores principales: Markt, Rudolf, Mayr, Markus, Peer, Evelyn, Wagner, Andreas O., Lackner, Nina, Insam, Heribert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471725/
https://www.ncbi.nlm.nih.gov/pubmed/34578246
http://dx.doi.org/10.3390/pathogens10091215
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author Markt, Rudolf
Mayr, Markus
Peer, Evelyn
Wagner, Andreas O.
Lackner, Nina
Insam, Heribert
author_facet Markt, Rudolf
Mayr, Markus
Peer, Evelyn
Wagner, Andreas O.
Lackner, Nina
Insam, Heribert
author_sort Markt, Rudolf
collection PubMed
description SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of the storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C for up to 9 days had no significant effect, while storage at −20 °C led to a significant reduction in gene copy numbers.
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spelling pubmed-84717252021-09-28 Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature Markt, Rudolf Mayr, Markus Peer, Evelyn Wagner, Andreas O. Lackner, Nina Insam, Heribert Pathogens Brief Report SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of the storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C for up to 9 days had no significant effect, while storage at −20 °C led to a significant reduction in gene copy numbers. MDPI 2021-09-18 /pmc/articles/PMC8471725/ /pubmed/34578246 http://dx.doi.org/10.3390/pathogens10091215 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Markt, Rudolf
Mayr, Markus
Peer, Evelyn
Wagner, Andreas O.
Lackner, Nina
Insam, Heribert
Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title_full Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title_fullStr Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title_full_unstemmed Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title_short Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
title_sort detection and stability of sars-cov-2 fragments in wastewater: impact of storage temperature
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471725/
https://www.ncbi.nlm.nih.gov/pubmed/34578246
http://dx.doi.org/10.3390/pathogens10091215
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