Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days

SIMPLE SUMMARY: Protein is considered as the most expensive nutrient in animal diet and requires exogenous proteolytic enzymes for proper digestion in all living organisms. Thus, the present study evaluated different crude protein levels (CP) with or without exogenous protease in broilers diet over 15–28 days on their growth and nutrient digestibility. Based on the obtained findings, it is recommended a reduction in protein contents of diets from 21 to 19% when the protease enzyme is supplemented, to produce a beneficial impact on the growth and digestibility of broilers and also to reduce feeding costs. ABSTRACT: In this trial, a 3 × 2 factorial design with different dietary crude protein levels (CP, 17, 19 and 21%) and two levels of exogenous protease (0 and 30,000 IU/kg) was used. A total of 540 two-week old broilers (Ross-308) was randomly allocated to experimental diets over 15–28 days of age. The interaction between dietary protein levels and enzyme supplementation showed that body weight gain was significantly (p < 0.05) higher in birds fed CP-19 (1114.7 g) and CP-21 (1108.8 g) with enzymes supplementation. Feed intake was higher (p < 0.05) in broilers fed with CP-17 than CP-19 with supplementation of the protease enzyme. Results also revealed that the feed conversion ratio (FCR) was significantly (p < 0.05) improved in birds fed with CP-19 and CP-21 and protease supplementation. Total tract N retention was lower (p < 0.05) in birds fed CP-17 with no enzyme than the other dietary groups. Similarly, the gross energy (GE) was significantly (p < 0.05) lower in birds fed CP-17 with or without the protease enzyme. Abdominal fat was higher (p < 0.05) in CP-17 (0.96%) without the protease enzyme. It was concluded that a diet at 19% CP with the protease enzyme improved the performance and nutrient digestibility in broilers over 15–28 days.