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Quantitative Approaches to Study Retinal Neurogenesis
The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems leve...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471905/ https://www.ncbi.nlm.nih.gov/pubmed/34572408 http://dx.doi.org/10.3390/biomedicines9091222 |
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author | Pérez-Dones, Diego Ledesma-Terrón, Mario Míguez, David G. |
author_facet | Pérez-Dones, Diego Ledesma-Terrón, Mario Míguez, David G. |
author_sort | Pérez-Dones, Diego |
collection | PubMed |
description | The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems level. Here, we review the ongoing transition toward a full four-dimensional characterization of the developing vertebrate retina, focusing on the challenges at the experimental, image acquisition, image processing and quantification. Using the developing zebrafish retina, we illustrate how quantitative data extracted from these type of highly dense, three-dimensional tissues depend strongly on the image quality, image processing and algorithms used to segment and quantify. Therefore, we propose that the scientific community that focuses on developmental systems could strongly benefit from a more detailed disclosure of the tools and pipelines used to process and analyze images from biological samples. |
format | Online Article Text |
id | pubmed-8471905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84719052021-09-28 Quantitative Approaches to Study Retinal Neurogenesis Pérez-Dones, Diego Ledesma-Terrón, Mario Míguez, David G. Biomedicines Review The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems level. Here, we review the ongoing transition toward a full four-dimensional characterization of the developing vertebrate retina, focusing on the challenges at the experimental, image acquisition, image processing and quantification. Using the developing zebrafish retina, we illustrate how quantitative data extracted from these type of highly dense, three-dimensional tissues depend strongly on the image quality, image processing and algorithms used to segment and quantify. Therefore, we propose that the scientific community that focuses on developmental systems could strongly benefit from a more detailed disclosure of the tools and pipelines used to process and analyze images from biological samples. MDPI 2021-09-14 /pmc/articles/PMC8471905/ /pubmed/34572408 http://dx.doi.org/10.3390/biomedicines9091222 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pérez-Dones, Diego Ledesma-Terrón, Mario Míguez, David G. Quantitative Approaches to Study Retinal Neurogenesis |
title | Quantitative Approaches to Study Retinal Neurogenesis |
title_full | Quantitative Approaches to Study Retinal Neurogenesis |
title_fullStr | Quantitative Approaches to Study Retinal Neurogenesis |
title_full_unstemmed | Quantitative Approaches to Study Retinal Neurogenesis |
title_short | Quantitative Approaches to Study Retinal Neurogenesis |
title_sort | quantitative approaches to study retinal neurogenesis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471905/ https://www.ncbi.nlm.nih.gov/pubmed/34572408 http://dx.doi.org/10.3390/biomedicines9091222 |
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