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Quantitative Approaches to Study Retinal Neurogenesis

The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems leve...

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Autores principales: Pérez-Dones, Diego, Ledesma-Terrón, Mario, Míguez, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471905/
https://www.ncbi.nlm.nih.gov/pubmed/34572408
http://dx.doi.org/10.3390/biomedicines9091222
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author Pérez-Dones, Diego
Ledesma-Terrón, Mario
Míguez, David G.
author_facet Pérez-Dones, Diego
Ledesma-Terrón, Mario
Míguez, David G.
author_sort Pérez-Dones, Diego
collection PubMed
description The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems level. Here, we review the ongoing transition toward a full four-dimensional characterization of the developing vertebrate retina, focusing on the challenges at the experimental, image acquisition, image processing and quantification. Using the developing zebrafish retina, we illustrate how quantitative data extracted from these type of highly dense, three-dimensional tissues depend strongly on the image quality, image processing and algorithms used to segment and quantify. Therefore, we propose that the scientific community that focuses on developmental systems could strongly benefit from a more detailed disclosure of the tools and pipelines used to process and analyze images from biological samples.
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spelling pubmed-84719052021-09-28 Quantitative Approaches to Study Retinal Neurogenesis Pérez-Dones, Diego Ledesma-Terrón, Mario Míguez, David G. Biomedicines Review The study of the development of the vertebrate retina can be addressed from several perspectives: from a purely qualitative to a more quantitative approach that takes into account its spatio-temporal features, its three-dimensional structure and also the regulation and properties at the systems level. Here, we review the ongoing transition toward a full four-dimensional characterization of the developing vertebrate retina, focusing on the challenges at the experimental, image acquisition, image processing and quantification. Using the developing zebrafish retina, we illustrate how quantitative data extracted from these type of highly dense, three-dimensional tissues depend strongly on the image quality, image processing and algorithms used to segment and quantify. Therefore, we propose that the scientific community that focuses on developmental systems could strongly benefit from a more detailed disclosure of the tools and pipelines used to process and analyze images from biological samples. MDPI 2021-09-14 /pmc/articles/PMC8471905/ /pubmed/34572408 http://dx.doi.org/10.3390/biomedicines9091222 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pérez-Dones, Diego
Ledesma-Terrón, Mario
Míguez, David G.
Quantitative Approaches to Study Retinal Neurogenesis
title Quantitative Approaches to Study Retinal Neurogenesis
title_full Quantitative Approaches to Study Retinal Neurogenesis
title_fullStr Quantitative Approaches to Study Retinal Neurogenesis
title_full_unstemmed Quantitative Approaches to Study Retinal Neurogenesis
title_short Quantitative Approaches to Study Retinal Neurogenesis
title_sort quantitative approaches to study retinal neurogenesis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471905/
https://www.ncbi.nlm.nih.gov/pubmed/34572408
http://dx.doi.org/10.3390/biomedicines9091222
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