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Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its gen...

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Autores principales: Onihary, Alain Moïse, Razanajatovo, Iony Manitra, Rabetafika, Lydia, Bastaraud, Alexandra, Heraud, Jean-Michel, Rasolofo, Voahangy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472404/
https://www.ncbi.nlm.nih.gov/pubmed/34578294
http://dx.doi.org/10.3390/v13091713
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author Onihary, Alain Moïse
Razanajatovo, Iony Manitra
Rabetafika, Lydia
Bastaraud, Alexandra
Heraud, Jean-Michel
Rasolofo, Voahangy
author_facet Onihary, Alain Moïse
Razanajatovo, Iony Manitra
Rabetafika, Lydia
Bastaraud, Alexandra
Heraud, Jean-Michel
Rasolofo, Voahangy
author_sort Onihary, Alain Moïse
collection PubMed
description White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.
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spelling pubmed-84724042021-09-28 Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016) Onihary, Alain Moïse Razanajatovo, Iony Manitra Rabetafika, Lydia Bastaraud, Alexandra Heraud, Jean-Michel Rasolofo, Voahangy Viruses Article White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry. MDPI 2021-08-28 /pmc/articles/PMC8472404/ /pubmed/34578294 http://dx.doi.org/10.3390/v13091713 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Onihary, Alain Moïse
Razanajatovo, Iony Manitra
Rabetafika, Lydia
Bastaraud, Alexandra
Heraud, Jean-Michel
Rasolofo, Voahangy
Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title_full Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title_fullStr Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title_full_unstemmed Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title_short Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
title_sort genotype diversity and spread of white spot syndrome virus (wssv) in madagascar (2012–2016)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472404/
https://www.ncbi.nlm.nih.gov/pubmed/34578294
http://dx.doi.org/10.3390/v13091713
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