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From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi

Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium refe...

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Autores principales: Joly, Pierre, Calteau, Alexandra, Wauquier, Aurélie, Dumas, Rémi, Beuvin, Mylène, Vallenet, David, Crovadore, Julien, Cochard, Bastien, Lefort, François, Berthon, Jean-Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472612/
https://www.ncbi.nlm.nih.gov/pubmed/34576819
http://dx.doi.org/10.3390/microorganisms9091924
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author Joly, Pierre
Calteau, Alexandra
Wauquier, Aurélie
Dumas, Rémi
Beuvin, Mylène
Vallenet, David
Crovadore, Julien
Cochard, Bastien
Lefort, François
Berthon, Jean-Yves
author_facet Joly, Pierre
Calteau, Alexandra
Wauquier, Aurélie
Dumas, Rémi
Beuvin, Mylène
Vallenet, David
Crovadore, Julien
Cochard, Bastien
Lefort, François
Berthon, Jean-Yves
author_sort Joly, Pierre
collection PubMed
description Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species Bacillus velezensis (synonym B. amyloliquefaciens subsp. plantarum or B. methylotrophicus), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other Bacillus spp. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent.
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spelling pubmed-84726122021-09-28 From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi Joly, Pierre Calteau, Alexandra Wauquier, Aurélie Dumas, Rémi Beuvin, Mylène Vallenet, David Crovadore, Julien Cochard, Bastien Lefort, François Berthon, Jean-Yves Microorganisms Article Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species Bacillus velezensis (synonym B. amyloliquefaciens subsp. plantarum or B. methylotrophicus), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other Bacillus spp. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent. MDPI 2021-09-10 /pmc/articles/PMC8472612/ /pubmed/34576819 http://dx.doi.org/10.3390/microorganisms9091924 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Joly, Pierre
Calteau, Alexandra
Wauquier, Aurélie
Dumas, Rémi
Beuvin, Mylène
Vallenet, David
Crovadore, Julien
Cochard, Bastien
Lefort, François
Berthon, Jean-Yves
From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title_full From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title_fullStr From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title_full_unstemmed From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title_short From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi
title_sort from strain characterization to field authorization: highlights on bacillus velezensis strain b25 beneficial properties for plants and its activities on phytopathogenic fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472612/
https://www.ncbi.nlm.nih.gov/pubmed/34576819
http://dx.doi.org/10.3390/microorganisms9091924
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