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Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses

The VP60 capsid protein from rabbit haemorrhagic disease virus (RHDV), the causative agent of one of the most economically important disease in rabbits worldwide, forms virus-like particles (VLPs) when expressed using heterologous protein expression systems such as recombinant baculovirus, yeasts, p...

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Autores principales: Dalton, Kevin P., Alvarado, Carmen, Reytor, Edel, del Carmen Nuñez, Maria, Podadera, Ana, Martínez-Alonso, Diego, Alonso, Jose Manuel Martin, Nicieza, Ines, Gómez-Sebastián, Silvia, Dalton, Romy M., Parra, Francisco, Escribano, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472679/
https://www.ncbi.nlm.nih.gov/pubmed/34579243
http://dx.doi.org/10.3390/vaccines9091005
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author Dalton, Kevin P.
Alvarado, Carmen
Reytor, Edel
del Carmen Nuñez, Maria
Podadera, Ana
Martínez-Alonso, Diego
Alonso, Jose Manuel Martin
Nicieza, Ines
Gómez-Sebastián, Silvia
Dalton, Romy M.
Parra, Francisco
Escribano, José M.
author_facet Dalton, Kevin P.
Alvarado, Carmen
Reytor, Edel
del Carmen Nuñez, Maria
Podadera, Ana
Martínez-Alonso, Diego
Alonso, Jose Manuel Martin
Nicieza, Ines
Gómez-Sebastián, Silvia
Dalton, Romy M.
Parra, Francisco
Escribano, José M.
author_sort Dalton, Kevin P.
collection PubMed
description The VP60 capsid protein from rabbit haemorrhagic disease virus (RHDV), the causative agent of one of the most economically important disease in rabbits worldwide, forms virus-like particles (VLPs) when expressed using heterologous protein expression systems such as recombinant baculovirus, yeasts, plants or mammalian cell cultures. To prevent RHDV dissemination, it would be beneficial to develop a bivalent vaccine including both RHDV GI.1- and RHDV GI.2-derived VLPs to achieve robust immunisation against both serotypes. In the present work, we developed a strategy of production of a dual-serving RHDV vaccine co-expressing the VP60 proteins from the two RHDV predominant serotypes using CrisBio technology, which uses Tricholusia ni insect pupae as natural bioreactors, which are programmed by recombinant baculovirus vectors. Co-infecting the insect pupae with two baculovirus vectors expressing the RHDV GI.1- and RHDV GI.2-derived VP60 proteins, we obtained chimeric VLPs incorporating both proteins as determined by using serotype-specific monoclonal antibodies. The resulting VLPs showed the typical size and shape of this calicivirus as determined by electron microscopy. Rabbits immunised with the chimeric VLPs were fully protected against a lethal challenge infection with the two RHDV serotypes. This study demonstrates that it is possible to generate a dual cost-effective vaccine against this virus using a single production and purification process, greatly simplifying vaccine manufacturing.
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spelling pubmed-84726792021-09-28 Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses Dalton, Kevin P. Alvarado, Carmen Reytor, Edel del Carmen Nuñez, Maria Podadera, Ana Martínez-Alonso, Diego Alonso, Jose Manuel Martin Nicieza, Ines Gómez-Sebastián, Silvia Dalton, Romy M. Parra, Francisco Escribano, José M. Vaccines (Basel) Article The VP60 capsid protein from rabbit haemorrhagic disease virus (RHDV), the causative agent of one of the most economically important disease in rabbits worldwide, forms virus-like particles (VLPs) when expressed using heterologous protein expression systems such as recombinant baculovirus, yeasts, plants or mammalian cell cultures. To prevent RHDV dissemination, it would be beneficial to develop a bivalent vaccine including both RHDV GI.1- and RHDV GI.2-derived VLPs to achieve robust immunisation against both serotypes. In the present work, we developed a strategy of production of a dual-serving RHDV vaccine co-expressing the VP60 proteins from the two RHDV predominant serotypes using CrisBio technology, which uses Tricholusia ni insect pupae as natural bioreactors, which are programmed by recombinant baculovirus vectors. Co-infecting the insect pupae with two baculovirus vectors expressing the RHDV GI.1- and RHDV GI.2-derived VP60 proteins, we obtained chimeric VLPs incorporating both proteins as determined by using serotype-specific monoclonal antibodies. The resulting VLPs showed the typical size and shape of this calicivirus as determined by electron microscopy. Rabbits immunised with the chimeric VLPs were fully protected against a lethal challenge infection with the two RHDV serotypes. This study demonstrates that it is possible to generate a dual cost-effective vaccine against this virus using a single production and purification process, greatly simplifying vaccine manufacturing. MDPI 2021-09-09 /pmc/articles/PMC8472679/ /pubmed/34579243 http://dx.doi.org/10.3390/vaccines9091005 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dalton, Kevin P.
Alvarado, Carmen
Reytor, Edel
del Carmen Nuñez, Maria
Podadera, Ana
Martínez-Alonso, Diego
Alonso, Jose Manuel Martin
Nicieza, Ines
Gómez-Sebastián, Silvia
Dalton, Romy M.
Parra, Francisco
Escribano, José M.
Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title_full Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title_fullStr Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title_full_unstemmed Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title_short Chimeric VLPs Bearing VP60 from Two Serotypes of Rabbit Haemorrhagic Disease Virus Are Protective against Both Viruses
title_sort chimeric vlps bearing vp60 from two serotypes of rabbit haemorrhagic disease virus are protective against both viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472679/
https://www.ncbi.nlm.nih.gov/pubmed/34579243
http://dx.doi.org/10.3390/vaccines9091005
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